逆神经走行方向探寻并摘取大鼠背根神经节的改进方法  

An improved method of exploring and extracting dorsal root ganglions in rats by reversely along the nerve traveling pathway

作　　者：马理元 姜劲挺[1,2] 张伦广 李祥雨 郑吉元 徐欣[1] 王强强 MA Liyuan;JIANG Jingting;ZHANG Lunguang;LI Xiangyu;ZHENG Jiyuan;XU Xin;WANG Qiangqiang(Gansu University of Chinese Medicine,Lanzhou 730000,China;Affiliated Hospital of Gansu University of Chinese Medicine,Lanzhou 730000;Lanzhou University Second Hospital,Lanzhou 730000)

机构地区：[1]甘肃中医药大学,兰州730000 [2]甘肃中医药大学附属医院,兰州730000 [3]兰州大学第二医院,兰州730000

出　　处：《中国比较医学杂志》2018年第3期95-97,共3页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(编号:81660799);甘肃省教育厅(编号:BH2010-039)

摘　　要：目的探寻一种快捷、便利、高效的摘取大鼠背根神经节(dorsal root ganglion,DRG)新方法——逆神经走行方向探寻并摘取DRG。方法分别采用通过打开椎间孔的传统方法和新方法摘取DRG,比较两种取材方法所消耗的时间和摘取的完整DRG数量。结果采用传统方法每只大鼠摘取L3~5节段完整背根神经节数量为(3.08±1.31)个,平均每个DRG耗时(5.58±1.21)min;采用新方法每只大鼠摘取L3~5节段完整背根神经节数量为(4.29±1.08)个,平均每个DRG耗时(1.69±0.91)min,与传统方法相比差异有显著性(P<0.05)。结论逆神经走行方向探寻并摘取DRG这一新方法可更高效地摘取得完整的大鼠背根神经节,为实验后期背根神经节细胞的培养和形态学研究提供了更为可靠的组织材料。Objective To explore a new efficient extraction method of dorsal root ganglions(DRGs)of rats by exploring and extracting DRGs reversely along the nerve traveling pathway.Methods The DRGs were extracted by the traditional method of opening the intervertebral foramina and the new method,extracting DRGs reversely along the nerve traveling pathway,respectively.The time consuming and the number of intact DRGs obtained with these two method were compared.Results The number of intact DRGs(L 3-5 segments,both sides)extracted from each rat with the traditional method was(3.08±1.31),and the average time consuming of each DRG was(5.58±1.21)min.As for the new method,the number of intact DRGs extracted from each rat was(4.29±1.08),and the average time consuming was(1.69±0.91)min,significantly better than that of the traditional method(P<0.05 for both).Conclusions The new method of exploring and extracting DRGs reversely along the nerve traveling pathway is more efficient for obtaining intact DRGs of rats,providing more useful tissue materials for subsequent culture and morphological studies of DRG cells.

关 键 词：大鼠 背根神经节 取材方法 

分 类 号：R-33[医药卫生]