黄鲫蛋白抗菌肽-纳米氧化锌络合物对小鼠肠黏膜氧化性的影响  

Effects of the Nanocomposite of Half-fin Anchovy Antibacterial Peptides and Zinc Oxide Nanoparticles on Oxidative Status of Intestinal Mucosa in Mice

作　　者：谷盈睿 韦荣编 宋茹 GU Ying-rui;WEI Rong-bian;SONG Ru(Food and Pharmaceutical College, Zhejiang Ocean University, Zhoushan,Zhejiang 316022;Marine Science and Technology, Zhejiang Ocean University, Zhoushan,Zhejiang 316022)

机构地区：[1]浙江海洋大学食品与医药学院,浙江舟山316022 [2]浙江海洋大学海洋科学与技术学院,浙江舟山316022

出　　处：《安徽农业科学》2018年第9期141-142,160,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省公益技术研究农业项目(2016C32066);浙江省自然科学基金一般项目(LY15C200018)

摘　　要：[目的]研究黄鲫蛋白抗菌肽-纳米氧化锌络合物对肠黏膜氧化作用的影响。[方法]以小鼠为动物模型,透射电镜分析显示,黄鲫蛋白抗菌肽-纳米氧化锌络合物微观为形状不规则的聚合物,将该聚合物注射到小鼠空肠中吸收30 min,分别测定肠黏膜的髓过氧化物酶(MPO)和谷胱甘肽过氧化物酶(GPx)活性,脂质过氧化物(LPO)和丙二醛(MDA)含量变化。[结果]试验表明,黄鲫蛋白抗菌肽-纳米氧化锌络合物显著提高了MPO活性,但是GPx活性则显著地降低;同样,在脂质氧化程度上,LPO含量显著地降低,而MDA含量则是显著地增加。[结论]该研究说明,黄鲫蛋白抗菌肽-纳米氧化锌络合物能改变小肠黏膜关键酶MPO和GPx活性,但是不同酶对其敏感性不同,MPO活性增强可能与LPO含量降低有关,而GPx活性降低则导致了MDA含量增加。[Objective]To study the effect of the antibacterial peptide-nanoscale Zinc Oxide complex on the intestinal mucosal oxidation of the crucian carp.[Method]Mice were used as animal models,the effects of the nanocomposite of half-fin anchovy antibacterial peptides and zinc oxide nanoparticles on the oxidative status of intestinal mucosa were investigated in mice model.Results of transmission electron microscope analysis showed that the microstructure of the nanocomposite of half-fin anchovy antibacterial peptides and zinc oxide nanoparticles was irregular polymer.This nanocomposite was injected into mice jejunum and adsorbed for30min,then the activities of myeloperoxidase(MPO)and glutathione peroxidase(GPx)were determined,as well as the contents of LPO and MDA were measured.[Result]Results showed a significant increase of MPO and decrease of GPx for jejunum mucosa.The content of LPO was dramatically decreased.In contrast,the content of MDA was significantly increased.[Conclusion]This study showed that yellow carp protein nano antibacterial peptide-Zinc Oxide complex can change the intestinal mucosal key enzymes MPO and GPx activity,but the sensitivity of different enzymes,increased the activity of MPO and LPO may decrease the content related,while GPx activity decreased resulted in the increase of MDA content.

关 键 词：黄鲫蛋白抗菌肽 纳米氧化锌 聚合物 微观特征 肠黏膜 氧化性 

分 类 号：TS201.2[轻工技术与工程—食品科学]