PCR-DGGE法分析海参花酶解物对小鼠肠道菌群的影响  

The Effects of Feeding Enzymatic Hydrolysate of Sea Cucumber Gonad on Intestinal Microbiota of Mice by PCR-DGGE

作　　者：白雅婷 吴国宏[2] 许智海 石林 何传波[2] 熊何健[2] 马英[1] BAI Yating;WU Guohong;XU Zhihai;SHI Lin;HE Chuanbo;XIONG Hejian;MA Ying(Fisheries College, Jimei University, Xiamen 361021, China;School of Bioengineering, Jimei University, Xiamen 361021, China;Ocean and Fishery Bureau of Zhangpu County, Zhangpu 363200, China)

机构地区：[1]集美大学水产学院,福建厦门361021 [2]集美大学生物工程学院,福建厦门361021 [3]漳浦县海洋与渔业局,福建漳浦363200

出　　处：《集美大学学报（自然科学版）》2018年第1期8-16,共9页Journal of Jimei University：Natural Science

基　　金：国家海洋局海洋公益性行业科研专项(201405016);福建省科技引导性项目(2016N0022);厦门市科技计划项目(3502Z20153014);集美大学特级大学生创新创业训练计划项目(201610390060)

摘　　要：为研究海参花酶解物对小鼠肠道微生物的影响,对灌喂生理盐水、海参花酶解物(低、中、高剂量)、抗坏血酸的小鼠粪便样品采用PCR-DGGE技术勘察其微生物的群落结构及动态变化。结果表明:小鼠粪便样品中的微生物主要有拟杆菌门(Bacteroidetes)、变形菌门(Probeobecteria)、厚壁菌门(Firmicutes)、脱铁杆菌门(Deferribacteres)以及未鉴定的细菌克隆子等,其中拟杆菌门、变形菌门为优势类群。随着实验的进行,灌喂海参花酶解物样品的DGGE图谱逐渐聚类在一起,并与空白组分开,且未鉴定克隆子的比例增加。不同的海参花酶解物浓度对小鼠肠道微生物群落影响也不同。随着灌喂时间的延长,灌喂抗坏血酸组对小鼠肠道微生物的影响逐渐显现,并与灌喂海参花的某些样品聚在一起。说明灌喂海参花酶解物后改变了小鼠肠道菌群结构。The paper studied the effects of feeding enzymatic hydrolysate of sea cucumber gonad on intestinal microbiota of mice by PCR-DGGE technique.Fifty mice were randomly and evenly divided into5groups,including one control group(feeding normal saline),three treatment groups(feeding low-,middle-,and high-dose of hydrolysate,respectively)and one Vc feeding group.The results indicated that the microbiota in mice stool samples mainly included Bacteroidetes,Probeobecteria,Firmicutes,Deferribacteres and Unidentified clones,in which the Bacteroidetes and Probeobecteria were dominant groups.With the experiment going on,the DGGE profiles of treatment groups clustered together and separated with the control group,and the unidentified clones in treatments groups increased.The effects of different feeding dose of enzymatic hydrolysate on the intestinal microbiota of mice were different.With the feeding time extension,the effect of Vc feeding group was becoming apparent,and the Vc feeding group clustered with some of the hydrolysate feeding samples.The results indicated that feeding enzymatic hydrolysate of sea cucumber gonad could alter the intestinal microbiota of mice.

关 键 词：海参花酶解物 肠道菌群 16SRRNA基因 PCR-DGGE 小鼠 

分 类 号：S917.4[农业科学—水产科学]