电强化磁固定化菌株Cupriavidus sp.JS耦合体系治理含苯酚废水  

The treatment of phenolic wastewater by electrodes assisted magnetically immobilized cells of strain Cupriavidus sp.JS

作　　者：季翔宇 徐进 刘佳鑫[1] 李楠[1,2] 时胜男[1] JI Xiangyu;XU Jin;LIU Jiaxin;LI Nan;SHI Shengnan(School of Life Science,Liaoning Normal University,Dalian Liaoning 116081;Liaoning Ocean and Fisheries Science Research Institute,Dalian Liaoning 116023)

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081 [2]辽宁省海洋水产科学研究院,辽宁大连116023

出　　处：《环境污染与防治》2018年第3期247-251,共5页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.51508259);辽宁省大学生创新创业训练计划项目(No.201710165000234);辽宁省海洋与渔业厅科研项目(No.201518)

摘　　要：为进一步提高含苯酚废水处理效果,从活性污泥中分离纯化得到1株具有高效苯酚降解能力的菌株Cupriavidus sp.JS,并构建了电强化磁固定化菌株Cupriavidus sp.JS耦合体系(以下简称耦合体系)。在好氧条件下比较耦合体系、磁固定化细胞及电极体系中的苯酚降解率及苯酚羟化酶活性,同时,考察重复利用中耦合体系的苯酚降解率、苯酚羟化酶活性及菌株Cupriavidus sp.JS浓度的变化。结果表明,耦合体系的最佳降解电压为1.0 V,此时的苯酚羟化酶活性最大,耦合体系对苯酚的降解率(100.0%)明显高于磁固定化细胞(90.6%)和电极体系(2.1%)之和,表明在耦合体系中生物降解和电极降解间存在耦合协同作用。同时,耦合体系中苯酚降解率、苯酚羟化酶活性及菌株Cupriavidus sp.JS浓度随着重复利用次数的增加而逐渐升高。Strain Cupriavidus sp.JS with phenol degrading ability was isolated from activated sludge and the electrodes assisted magnetically immobilized cells of strain Cupriavidus sp.JS(short for coupling system)was designed in this study to enhance the treatment performance of phenolic wastewater.The degradation rates of phenol and phenol hydroxylase activity by the coupling system,single magnetically immobilized cells and electrodes system were compared.Meanwhile,the degradation rate of phenol,phenol hydroxylase activity and concentration of the strain in the reused coupling system were also investigated.The results indicated that the degradation rate of phenol by the coupling system was 100.0%,which was significantly higher than the sum of single magnetically immobilized cells(90.6%)and electrodes system(2.1%)at the optimal voltage of 1.0 V.It suggested that there was a coupling synergy effect between the biodegradation and electrode degradation.The degradation rate of phenol,phenol hydroxylase activity and concentration of the strain in coupling system increased gradually with the increase of reuse times.

关 键 词：苯酚 电强化 磁固定化细胞 苯酚羟化酶 

分 类 号：X172[环境科学与工程—环境科学] X703