miRNA-203联合TSGF、CEA检测在胃癌诊断中的应用  

Application of miRNA-203 combined with TSGF and CEA detection in diagnosis of gastric cancer

作　　者：殷立奎 徐广晓 朱新兴 李守勇 YIN Likui;XU Guangxiao;ZHU Xinxing;LI Shouyong(Department of Clinical Laboratory Dongying Municipal People′s Hospital,Dongying,Shandong 257091,China;Operation Room,Dongying Municipal People′s Hospital,Dongying,Shandong 257091,China)

机构地区：[1]山东省东营市人民医院检验科,257091 [2]山东省东营市人民医院手术室,257091

出　　处：《检验医学与临床》2018年第6期821-824,共4页Laboratory Medicine and Clinic

摘　　要：目的分析微小核糖核酸-203(miRNA-203)联合肿瘤生长因子(TSGF)、癌胚抗原(CEA)检测在胃癌诊断中的应用。方法选择2015年7月至2016年7月来该院就诊的40例胃癌患者为观察组,选择同期30例健康志愿者为对照组,分析研究对象的外周血清CEA、miRNA-203、TSGF水平。结果观察组患者miRNA-203相对表达量为(0.26±0.16)U/mL,明显低于对照组的(0.84±0.09)U/mL(P<0.05);观察组TSGF、CEA表达水平及阳性率明显高于对照组(P<0.05)。miRNA-203相对表达量随分期递增而减少(F=5.60,P=0.007 5)。TSGF、CEA表达水平随分期递增而增加(P<0.05);不同分期CEA阳性率比较差异有统计学意义(P<0.05),而不同分期TSGF阳性率差异无统计学意义(P>0.05)。miRNA-203联合TSGF、CEA检测的敏感度为95.00%,明显高于单项检测或者2项指标联合检测结果,差异有统计学意义(P<0.05)。miRNA-203联合TSGF、CEA检测的诊断符合率为97.50%,特异度为90.00%,优于单项或者两项联合结果(P<0.05)。结论采用miRNA-203联合TSGF、CEA检测,可以及时准确诊断胃癌,具有较高的特异度与诊断符合率,在临床上有较高的诊断价值。Objective To investigate the application of miRNA-203 combined with TSGF and CEA detection in the diagnosis of gastric cancer.Methods Forty patients with gastric cancer treated in this hospital from July 2015 to July 2016 were selected as the observation group and contemporaneous 30 healthy volunteers were selected as the control group.The peripheral serum CEA,miRNA-203 and TSGF in the study subjects were analyzed.Results The relative expression level of miRNA-203 in the observation group was(0.26±0.16)U/mL,which was significantly lower than(0.84±0.09)U/mL in the control group(P<0.05).The expression levels and positive rates of TSGF and CEA were significantly higher than those in the control group(P<0.05).The relative expression level of miRNA-203 was decreased with the stage increase(F=5.60,P=0.007 5).The expression levels of TSGF and CEA were increased with the stage increase(P<0.05);the CEA positive rate had statistical difference among different stages(P<0.05),while the positive rate of TSGF had no statistical difference among different stages(P>0.05).The sensitivity of miRNA-203 combined with TSGF and CEA detection was 95.00%,which was significantly higher than that of single index or combined 2-index combined detection,the difference was statistically significant(P<0.05).The diagnosis coincidence rate of miRNA-203 combined with TSGF and CEA detection was 97.50%and the specificity was 90.00%,which were better than those in single index detection or two-index combined detection(P<0.05).Conclusion Adopting miRNA-203 combined with TSGF and CEA detection can timely and accurately diagnose gastric cancer with higher specificity and diagnostic coincidence rate and has higher diagnostic value in clinic.

关 键 词：微小核糖核酸-203 肿瘤生长因子 癌胚抗原 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]