高效液相色谱法同时测定六味补血胶囊中没食子酸、芍药苷和藁本内酯含量  

Simultaneous Determination of Gallic Acid,Paeoniflorin and Ligustilide in Liuwei Buxue Capsules by HPLC

作　　者：关潇滢 曾利娜 胡晓谕 温晓丽 赵渝 肖丽和 Guan Xiaoying;Zeng Lina;Hu Xiaoyu;Wen Xiaoli;Zhao Yu;Xiao Lihe(Shenzhen Institute for Drug Control,Shenzhen,Guangdong,China518057;Shenzhen Key Laboratory of Drug Quality Standard Research,Shenzhen,Guangdong,China518057;Shenyang Pharmaceutical University,Shenyang,Liaoning,China110016)

机构地区：[1]广东省深圳市药品检验研究院,广东深圳518057 [2]深圳药品质量标准研究重点实验室,广东深圳518057 [3]沈阳药科大学,辽宁沈阳110016

出　　处：《中国药业》2018年第5期13-15,共3页China Pharmaceuticals

基　　金：广东省深圳市科技计划项目[JCYJ20140722104416530]

摘　　要：目的建立测定六味补血胶囊中没食子酸、芍药苷和藁本内酯含量的高效液相色谱(HPLC)法。方法色谱柱采用Symmetry Shield RP_18 ODS柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,流速为1.0 mL/min,采用波长切换方式,检测波长没食子酸为280 nm,芍药苷为230 nm,藁本内酯为334 nm。结果没食子酸、芍药苷和藁本内酯质量浓度分别在0.046~0.910,0.049~0.970 g/L,2.1~52.3μg/mL范围内与峰面积线性关系良好,平均回收率分别为99.20%,98.79%,97.15%,RSD分别为0.29%,0.97%,0.81%(n=6)。结论该方法准确、可靠、重复性好,可用于六味补血胶囊的质量控制。Objective To establish an HPLC method for content determination of gallic acid,paeoniflorin and li/ustilide in Liuwei Buxue Capsules.Methods The Symmetry Shield RP18 0 DS column(250 m m x4.6 mm,5 pm)was adopted with gradient elution,the mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution,the flow rate was 1.0 mL/min,the mode of wavelength switching was used,the detection wavelength was 280 nm for gallic acid,230 nm for paeoniflorin and 334 nm for ligustilide.Results The linear ranges of gallic acid,paeoniflorin and ligustilide were 0.046-0.910 g/L,0.049-0.970 g/L,2.1-52.3 pg/mL.The average recoveries were 99.2 0%,98.7 9%,97.1 5%.RSDe were 0.2 9%,0.9 7%,0.8 1%(n=6).Conclusion The method is accurate,reliable and reproducible,which can be used for the quality control of Liuwei Buxue Capsules.

关 键 词：六味补血胶嚢 没食子酸 芍药苷 藁本内酯 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]