酶放大免疫测定技术法检测丙戊酸药物浓度的动态飘逸校正系数  

Dynamic Drift Correction Coefficient for Detection of Valproic Acid Concentration by EMIT

作　　者：周清武[1] Zhou Qingwu(Department of Pharmacy,Mianyang Central Hospital,Mianyang,Sichuan,China 621000)

机构地区：[1]四川省绵阳市中心医院药学部,四川绵阳621000

出　　处：《中国药业》2018年第5期22-25,共4页China Pharmaceuticals

摘　　要：目的探讨酶放大免疫测定技术法检测丙戊酸药物浓度的动态飘逸校正系数。方法以50μg/mL丙戊酸标准水溶液为质控样品,采用德国西门子全自动生化仪,分别在1个试剂盒的第48,108次测试时做质控检测,进行质控检测浓度动态飘逸指数函数的推拟。结果动态飘逸系数k=(1+0.000 66×n^(0.555 35))~n,动态飘逸校正系数k'=1/[(1+0.000 66×n^(0.555 35))~n],并以第1,20,31,97,120,135次质控样品检测浓度验证计算,相对误差在±6%以内。结论推拟得到的动态飘逸校正系数可用于丙戊酸质控检测浓度的动态校正,有利于丙戊酸血药浓度的准确检测,可减少丙戊酸检测系统不定期定标或质控产生的试剂浪费。To investigate the dynamic drift correction coefficient for detection of valproic acid concentration by enzyme multiplied immunoassay technique(IlMIT).Metliods 50(μg/mL valproic acid standard water solution was taken as the quality control sample.The automatic biochemical analyzer made in Germany Siemens was used to do the quality control test at the 48th,108th tests of a kit,in order to speculate the dynamic drift exponential function of the quality control detection concentration.Results The dynamic drift coefficient was obtained:A;=(1+0.000 66 x n0.555 35)n,and the dynamic correction coefficient was obtained:=1/[(1+0.000 66 x n0 555 35)n].The detection concentration of the 1st,20th,31st,97th 120th and 135th quality control samples were used to verify the calculation,and the relative errors were between-6%and 6%.Conclusion The obtained dynamic drift correction coefficient can be used for the dynamic correction of quality control concentration of valproic acid,which is conducive to the accurate detection of blood concentration of valproic acid,and it can reduce the waste of reagents produced by the irregular calibration or quality control of the valproic acid detection system.

关 键 词：酶放大免疫测定技术 丙戌酸 动态飘逸 校正系数 试剂瓶底效应 

分 类 号：R914.1[医药卫生—药物化学] R971.6[医药卫生—药学]