肝癌患者肝移植前外周血免疫表型分析  

Detection of Immune Cell Subsets of Peripheral Blood in Hepatocellular Carcinoma before Liver Transplantation

作　　者：邓小红 张倩倩[1,2,3] 蔡燕 欧阳涓[4] 陈茂根[1,2] 唐秀鑫[1,2] 何晓顺 黄俊琪[1,2] DENG Xiao-hong;ZHANG Qian-qian;CAI Yan;OUYANG Juan;CHEN Mao-gen;TANG Xiu-xin;HE Xiao-shun;HUANG Jun-qi(Department of Transplantation of the First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China;Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology//Guangdong Provincial International Cooperation Base of Science and Technology,Sun Yat-sen University,Guangzhou 510080,China;Institute of Immunology,Zhongshan School of Medicine,Guangzhou 510080,China;Department of Laboratory Medicine,the First Affiliated Hospital;Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学附属第一医院器官移植科,广东广州510080 [2]中山大学附属第一医院广东省器官捐献与移植免疫重点实验室//广东省器官移植国际科技合作基地,广东广州510080 [3]中山医学院免疫学研究所,广东广州510080 [4]中山大学附属第一医院检验科,广东广州510080

出　　处：《中山大学学报（医学版）》2018年第2期178-185,226,共9页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(31370870);广东省自然科学基金(S2013020013000);广东省科技计划项目(2013A020229003);广州市科技计划项目(201604020083);广东省器官捐献与移植免疫重点实验室建设项目(2013A061401007);广东省器官移植国际合作基地建设项目(2015B050501002)

摘　　要：【目的】探讨肝癌患者肝移植前外周血免疫细胞表型的特征。【方法】通过多色流式细胞术对6例准备接受肝移植的肝癌患者及6例健康对照组外周血中T细胞、B细胞、单核细胞亚群、树突状细胞(DC)及NK细胞进行免疫表型分析。【结果】与健康对照组比较,肝癌肝移植受者组患者外周血CD4^+PD-1^+T细胞、调节T细胞(CD4^+CD25^+CD39^+)、B细胞、浆母细胞(CD27^(high)CD38^(high)IgD^-IgM^-)、经典型单核细胞(CD14^(high)CD16^-)及未成熟NK细胞(CD3^-CD56^(high))均升高,差异具有统计学意义(P<0.05);边缘区B细胞(CD27^+IgD^+)、非类型转换B细胞(CD27^+CD38^(dim)IgM^+)、中间型单核细胞(CD14^(high)CD16^+)及NK细胞(CD3^-CD56^+)比例降低,差异具有统计学意义(P<0.05);其余细胞比例未见明显差异。【结论】等待肝移植的肝癌患者外周血免疫细胞的表型与健康人有差异,对监测患者移植后的免疫状态及指导免疫抑制剂的使用有重要作用。【Objective】To investigate the characteristics of immunophenotypes in hepatocellular carcinoma(HCC)before liver transplantation.【Methods】The immunophenotypes of T-,B-cells,monocytes,dendritic cells(DC)and NK-cells in peripheral blood from6HCC patient who were ready to have liver transplantation and6healthy volunteers were analyzed by multicolor flow cytometry.【Results】In the patients,the proportions of CD4+PD-1+T cells,Treg cell(CD4+CD25+CD39+T cells),CD19+B cells,Plasmablasts(CD27high CD38high IgD-IgM-),classical monocytes(CD14highCD16-)and mature NK-cells(CD3-CD56high)were all higher than those in the healthy controls(all P<0.05).However,marginal zone B cell(CD27+IgD+),Non-switched B cells(CD27+CD38dim IgM+),intermediate monocytes(CD14high CD16+)and immature NK-cells(CD3-CD56+)were lower than those in the healthy controls(all P<0.05).And there wasn’t any obvious difference in quantity being observed among other cell types.【Conclusion】There was difference in the immunophenotypes of immune cells in peripheral blood between HCC patients before liver transplantation and healthy people.And this finding exerts important effects on monitoring the immune status of the patients after liver transplantation and guiding the administrations of immunosuppressors.

关 键 词：肝癌 肝移植 免疫细胞亚群 多色流式细胞术 

分 类 号：R392.4[医药卫生—免疫学]