SAHA抑制乳腺癌ER受体阳性细胞系MCF-7细胞侵袭的形态学观察  

Morphological Study for the Cell Invasion of Breast Cancer Cell Line MCF-7 Treated by SAHA

作　　者：刘春阳 周伟强[2] LIU Chunyan;ZHOU Weiqiang(Grade 2016,Shenyang Medical College,Shenyang 110034,China;Shenyang Medical College,Key Laboratory of Environmental Pollution and Microecology of Liaoning Province)

机构地区：[1]沈阳医学院基础医学院,辽宁沈阳110034 [2]沈阳医学院基础医学院,辽宁省环境污染与微生态重点实验室

出　　处：《沈阳医学院学报》2018年第2期181-184,共4页Journal of Shenyang Medical College

基　　金：国家自然科学基金项目(No.81172509);辽宁省大学生创新创业科研基金项目(No.201710164000013,201710164000009)

摘　　要：目的:研究SAHA对乳腺癌雌激素受体阳性细胞系MCF-7细胞侵袭力的影响。方法:采用细胞增殖实验、细胞划痕、Transwell细胞侵袭力测定等实验从形态学角度观察SAHA对MCF-7细胞侵袭力的影响。结果:与Leptin处理组相比,SAHA明显抑制了MCF-7细胞内DNA复制能力,并限制了其游走性。Transwell实验也证实SAHA处理后的细胞侵袭性较对照组细胞明显减弱,细胞状态较差,呈星状和针刺样。结论:SAHA抑制了乳腺癌MCF-7细胞的侵袭力。Objective:To investigate the effect of SAHA on cell invasion of breast cancer cell line MCF-7.Method:Cell proliferation assays,cell scratching and Transwell assay were used to observe the effects of SAHA on cell invasion of MCF-7 cells.Results:Compared with Leptin treatment,SAHA significantly inhibited the DNA replication in the MCF-7 cells,and limited the ability of cell migration.Transwell array also confirmed that,after SAHA treatment,the cell invasion of MCF-7 cells was significantly weakened compared with control cells.The status of cell was poor,with star-shaped and needle-like bulges.Conclusion:SAHA inhibits cell invasion of breast cancer cell line MCF-7.

关 键 词：乳腺癌 MCF-7 细胞侵袭力 

分 类 号：R737.9[医药卫生—肿瘤]