芽孢杆菌Z-y3对Pb(Ⅱ)吸附特性及机理  

Characteristics and Mechanism of Pb(Ⅱ) Adsorption in a Bacillus sp. Strain Z-y3

作　　者：陈兰洲[1] 赵瑞雪[1] 武艳芳 邓松强[1] 梁亚楠 刘思瑶 Chen Lanzhou;Zhao Ruixue;Wu Yanfang;Deng Songqiang;Liang Yanan;Liu Siyao(School of Resource&Environmental Sciences,Hubei Research Center of Environment Remediation Technology,Wuhan University,Wuhan 430072,China;Wuhan Wenke Ecological Environment Company Ltd,Wuhan 430223,China)

机构地区：[1]武汉大学资源与环境科学学院,湖北省环境修复技术研究中心,武汉430072 [2]武汉文科生态环境有限公司,武汉430223

出　　处：《中南民族大学学报（自然科学版）》2018年第1期30-34,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31370421);湖北省技术创新重大专项(2016ACA162)

摘　　要：从重金属污染土壤中筛选出一株在酸性条件下对Pb(Ⅱ)有良好去除效果的芽孢杆菌Bacillus sp.(Z-y3),研究了其去除机理.结果发现:在酸性条件下,该菌能产碱,去除效果受环境p H影响较小且吸附迅速.通过XRD、SEM-EDS、FTIR、XPS等分析手段对该菌的吸附机理进行了研究,发现该菌株主要通过表面静电吸附、离子交换、产碱沉淀等方式,利用胞外蛋白多聚物、多糖的羟基和氨基结合重金属Pb(Ⅱ),并形成难溶晶体达到吸附重金属Pb(Ⅱ)的效果.该菌在较低酸性条件下对中低浓度的Pb(Ⅱ)有高效的去除效果,对铅污染土壤修复有一定的应用价值.Bacillus sp.named as Z-y3,which was isolated from heavy metal contaminated soil,had high efficiency of leadremoval capacity under acidic condition and its removal mechanism was studied.The results showed that this strain had goodremoval efficiency and was less affected by environmental pH condition.X-ray diffraction(XRD),scanning electronmicroscope and energy dispersive spectrometer(SEM-EDS),Fourier transform infrared(FTIR)and X-ray photoelectronspectroscopy(XPS)were used to investigate the adsorption mechanism.It was found that the surface electrostaticadsorption,ion exchange,precipitation by producing alkali played a major role in removal of Pb(II).Pb(II)wasadsorbed by means of the combination of extracellular polymeric substances,polysaccharide hydroxyl and amidogen with Pb(II)and forming insoluble crystal.The strain Z-y3had higher removal efficiency for low and medium concentration of Pb(II)under wide acidity condition,and thus had a potential application value for the restoration of environmental Pb(II)pollution.

关 键 词：生物吸附 重金属Pb(II) 吸附机理 芽孢杆菌 

分 类 号：X172[环境科学与工程—环境科学]