非小细胞肺癌组织PCK2的表达与预后的相关性研究  

The Correlationship Between Expression of PCK2 in Non-Small Cell Lung Cancer and Prognosis

作　　者：谭安隽[1] 阮丽波[1] 张利斌[2] 杨天睿[1] 张雯[3] TAN Anjun;RUAN Libo;ZHANG Libin;YANG Tianrui;ZHANG Wen(Cadre Health Care Department,The First People’s Hospital of Yunnan Province,Kunming Yunnan 650032,China;Department of Thoracic Surgery,The First People’s Hospital of Yunnan Province,Kunming Yunnan 650032,China;Basic Research Institute,The First People’s Hospital of Yunnan Province,Kunming Yunnan 650032,China)

机构地区：[1]云南省第一人民医院干部保健科,云南昆明650032 [2]云南省第一人民医院胸外科,云南昆明650032 [3]云南省第一人民医院基础研究所,云南昆明650032

出　　处：《中国继续医学教育》2018年第10期58-60,共3页China Continuing Medical Education

基　　金：云南省教育厅科学研究基金项目(2016ZDX009)

摘　　要：目的探讨非小细胞肺癌组织磷酸烯醇丙酮酸羧激酶2(PCK2)的表达与临床预后的相关性。方法选取2017年1—6月我院胸外科手术病理诊断为非小细胞肺癌的15例患者作为观察对象,取肺癌组织及距肿瘤边缘距离20 mm的远癌组织分别进行Real-time PCR检测。观察肺癌组织及远癌组织PCK2m RNA的表达情况,并分析PCK2m RNA与临床指标的相关性。随机选取我院病理科2014年1—12月的68例非小细胞肺癌的癌组织蜡块进行免疫组化,检测PCK2蛋白在癌组织中的表达情况,比较两年生存组和两年未生存组免疫组化评分。结果(1)肺癌组织与远癌组织比较PCK2m RNA的水平明显升高,差异具有统计学意义(P<0.01)。经多因素相关分析显示肺癌组织中PCK2m RNA与年龄、性别、是否吸烟、肿瘤分期、肿瘤组织类型均无相关性。(2)结果发现两年生存组的免疫组化评分低于两年未生存组,差异具有统计学意义(P<0.01)。结论 PCK2的激活状态与患者预后明显相关。Objective To investigate the correlation between the expression of phosphoenolpyruvate carboxyl kinase 2(PCK2)and the clinical prognosis in non-small cell lung cancer.Methods 15 patients with non-small cell lung cancer from January to June 2017 were selected from thoracic surgery in our hospital.Real-time PCR was used to detect lung cancer tissue and distant cancer tissues 20 mm from the edge of the tumor.To observe the expression of PCK2mRNA in lung cancer tissues and distant cancer tissues,and to analyze the correlation between PCK2mRNA and clinical indicators.68 cases of non-small cell lung cancer tissue was selected in our department from January to December 2014,we used immunohistochemical method to detect PCK2 protein expression in cancer tissue,compare immunohistochemical score between two years survival group and two years not survival group.Results(1)The level of PCK2mRNA was significantly increased in lung cancer tissues compared with distant cancer tissues,the difference was statistically significant(P<0.01).Multivariate analysis showed that there was no correlation between PCK2mRNA and age,sex,smoking,tumor staging and tumor tissue type in lung cancer tissues.(2)It was found that the immunohistochemistry score of the two years survival group was significantly lower than that in the two years not survival group,the difference was statistically significant(P<0.01).Conclusion The activation status of PCK2 is significantly correlated with the prognosis of patients.

关 键 词：非小细胞肺癌 磷酸烯醇丙酮酸羧激酶 免疫组化 预后 

分 类 号：R734[医药卫生—肿瘤]