三种生物活性陶瓷材料对iPS细胞增殖的影响  

The Impact of Three Bioactive Ceramic Materials on the Proliferation of iPS Cells

作　　者：吴琳 Wu Lin

机构地区：[1]浙江特殊教育职业学院,浙江杭州310023

出　　处：《科教文汇》2018年第9期70-71,共2页Journal of Science and Education

摘　　要：目的:比较三种生物活性陶瓷β-磷酸三钙β-TCP、镁橄榄石Mg2SiO4、斜顽灰石MgSiO3对iPS细胞增殖的影响,寻找适宜iPS细胞生长和增殖的生物陶瓷材料浸提液的浓度,从而选择合适的生物陶瓷材料将其应用于骨组织工程骨缺损的修复,进而应用于临床骨缺损的修复。结果:3种生物陶瓷材料都具有良好的生物相容性;3种生物陶瓷材料浸提液在一定浓度范围内(1/32~1/2倍过饱和生物陶瓷材料浸提液浓度)能促进iPS细胞的增殖;在一定浓度范围内(1/16~1/2倍过饱和生物陶瓷材料浸提液浓度)对iPS细胞增殖的促进作用MS>M2S>β-TCP,而在一定浓度范围内(1/64~1/32倍过饱和生物陶瓷材料浸提液浓度)对iPS细胞增殖的促进作用M2S>MS>β-TCP;最佳促进细胞增殖的浓度分别是1/32倍过饱和生物陶瓷材料浸提液浓度(M2S)和1/16倍过饱和生物陶瓷材料浸提液的浓度(MS、β-TCP)。Objective:To compare the impacts of three kinds of bioactive ceramic materials,β-tricalcium phosphate(β-TCP),magnesium olivine(Mg2SiO4)and oblique enstatite(MgSiO3),on the proliferation of iPS cells,and find the appropriate iPS cell proliferation biological extract concentration,in order to choose a suitable biological ceramic material and its application in bone tissue engineering repair of bone defect.Methods:In vitro amplification of iPS cells,three kinds of biological ceramic material extracts(each ceramic material extract has seven different concentrations),were added in iPS medium,using MTT method to observe different kinds,different concentrations of biological ceramic leaching liquid on iPS cell proliferation.

关 键 词：生物活性陶瓷 IPS细胞 增殖 

分 类 号：G712[文化科学—职业技术教育学]