雷州黑鸭FSHβ基因多态性与产蛋性能的关联分析  

FSHβ gene polymorphism and correlation between it and egg production performance in Leizhou black duck

作　　者：张少伟 崔红艳[1] 苏瑛[1] ZHANG Shao-wei;CUI Hong-yan;SU Ying(College of Agriculture,Guangdong Ocean University,Zhanjiang,Guangdong 524088,China)

机构地区：[1]广东海洋大学农学院,广东湛江524088

出　　处：《南方农业学报》2018年第1期142-147,共6页Journal of Southern Agriculture

基　　金：广东省科技计划项目(2014A020208123);广东省公益研究与能力建设专项项目(2017A020208066);湛江市现代农业重点项目(2015A03003);广东海洋大学研究生优秀学位论文培育项目(201610)

摘　　要：【目的】明确促卵泡激素β亚基(FSHβ)基因在雷州黑鸭群体中的遗传变异情况,为雷州黑鸭群的品种选育提供分子标记。【方法】采用PCR-SSCP对200羽雷州黑鸭FSHβ基因的外显子2(exon2)和内含子1(int1)进行多态性检测,通过测序筛查出多态性位点(SNP位点),同时分析不同基因型与雷州黑鸭产蛋性状间的相关性。【结果】在雷州黑鸭FSHβ基因的g.1103451A>T(int1)处发现一个单核苷酸突变位点(A→T),其存在3种基因型(AA型、AT型和TT型),对应的基因型频率分别为0.538、0.359和0.103。T等位基因频率(0.718)高于A等位基因频率(0.282),且g.1103451A>T位点在雷州黑鸭群体中符合Hardy-Weinberg平衡状态(P>0.05)。雷州黑鸭的开产日龄以TT型个体最早,其次是AT型个体,分别比AA型个体提前5.48和4.00 d,其差异均达显著水平(P<0.05,下同);在开产体重和300日龄产蛋数方面,也表现为TT和AT型个体显著高于AA型个体,且以TT型个体最高,分别为1299.31 g/羽和134.37枚;其他产蛋性状指标在不同基因型个体间的差异均不显著(P>0.05)。【结论】雷州黑鸭FSHβ基因int1存在一个SNP位点(g.1103451A>T),其与产蛋性能间存在显著相关性,主要对早期产蛋和生长发育具有促进作用,可作为雷州黑鸭品种选育的辅助选择分子标记。【Objective】The genetic variation of follicle stimulating hormoneβsubunit(FSHβ)gene in Leizhou black duck population was clarified to provide molecular markers for breeding of Leizhou black duck population.【Method】The polymorphisms of exon2 and intron 1(int1)of gene FSHβin 200 Leizhou black ducks were detected by PCR-SSCP,and the polymorphic sites(SNPs)were found and sequenced.The correlation between different genotypes and egg production traits of Leizhou black ducks was analyzed.【Result】A single nucleotide mutation site(A→T)was found at g.1103451A>T(int1)of gene FSHβin Leizhou black duck.There were three genotypes(AA,AT and TT).The corresponding genotype frequencies were 0.538,0.359 and 0.103 respectively.The frequency of T allele(0.718)was higher than that of allele A(0.282),and g.1103451A>T locus was in Hardy-Weinberg equilibrium(P>0.05)in Leizhou black duck population.Age at first egg of TT genotype was the earliest,followed by AT genotype,which were 5.48 and 4.00 d earlier than AA genotype respectively,and the difference reached significant level(P<0.05,the same below).In terms of weight at first egg and egg production at 300 day-age,TT and AT individuals were also significantly higher than those of AA individuals,and TT individuals were the highest(1299.31 g/feather and 134.37 eggs respectively).The rest of the egg production traits in each genotype were not significantly different between the individuals(P>0.05).【Conclusion】There is a SNP locus(g.1103451A>T)in int1 of Leizhou black duck gene FSHβ,which has significant correlation with its egg production performance.It mainly promotes early egg laying and growth,hence it may be assisted-selection molecular markers for Leizhou black duck breeding.

关 键 词：雷州黑鸭 FSHΒ基因 SNP位点 产蛋性能 关联分析 

分 类 号：S834.89[农业科学—畜牧学]