雄性中华狼蛛毒素多肽的分离及鉴定  

Isolation and identification of peptides derived from venom in male Lycosa sinensis

作　　者：唐兴 陈连水[1] 黄亚洲 段志贵[2] 刘中华[2] 梁宋平[2] TANG Xing;CHEN Lianshui;HUANG Yazhou;DUAN Zhigui;LIU Zhonghua;LIANG Songping(Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation,East China University of Technology,Nanchang 330013,China;The National&Local Joint Engineering Laboratory of Animal Peptide Drug Development,Hunan Normal University,Changsha 410081,China)

机构地区：[1]东华理工大学江西省质谱科学与仪器重点实验室,江西南昌330013 [2]湖南师范大学动物多肽药物国家地方联合工程实验室,湖南长沙410081

出　　处：《湖南农业大学学报（自然科学版）》2018年第1期71-76,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(31201718;31402025);长江学者和创新团队发展计划项目(IRT13054);江西省自然科学基金项目(20114BAB214015;20142BAB204011);江西省教育厅青年科学基金项目(GJJ12389);东华理工大学博士科研启动基金项目(DHBK1008);湖南省生物发育工程及新产品研发协同中心项目(20134486)

摘　　要：采用反向高效液相色谱对雄性中华狼蛛粗毒进行分离,再用MALDI–TOF–TOF质谱进行相对分子质量测定。结果表明:从雄性中华狼蛛粗毒中一共鉴定到195种毒素多肽组分,这些毒素多肽的相对分子质量为1 000~10 000,其中大多数多肽的相对分子质量为1 000~3 500和4 500~7 500。这些毒素多肽的相对分子质量的分布特征与已报道的其他蜘蛛的不同,这可能是因为不同种类蜘蛛的毒素成分具有不同的多样性特点。Peptides in crude venom from male Lycosa sinensis were isolated by means of reversed–phase high–performance liquid chromatography,and their relative mass in eluted fractions were analyzed through MALDI– TOF–TOF mass spectrometry.In total,195 peptides were detected out with the relative mass ranged from 1 000 to 10 000.The vast majority of these peptide mass fell in the ranges of 1 000–3 500 and 4 500–7 500,respectively.Its molecular weight distribution was far dissimilar from those of other spiders reported,which is probably because venom components might vary with spider species.

关 键 词：中华狼蛛 粗毒 多肽 相对分子质量 多样性 

分 类 号：Q959.226[生物学—动物学] R392.33[医药卫生—免疫学]