单酰甘油酰基转移酶2(MOGAT2)在翼状胬肉组织中的表达及意义  

Expression and significance of monoacylglycerol Oacyltransferase 2( MOGAT2) in pterygium

作　　者：秦柏[1] 张俊芳[1] 朱惠君[2] 杨梅[1] 杨铃[1] 钱晶晶[1] 梁舒[1] 吴玉宇[1] 陆宏[1] 黄剑飞[2] QIN Bai;ZHANG Jun-Fang;ZHU Hui-Jun;YANG Mei;YANG Ling;QIAN Jing-Jing;LIANG Shu;WU Yu-Yu;LU Hong;HUANG Jian-Fei(Department of Ophthalmology,Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China;Department of Pathology,Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China)

机构地区：[1]南通大学附属医院眼科,江苏省南通市226001 [2]南通大学附属医院病理科,江苏省南通市226001

出　　处：《眼科新进展》2018年第2期156-160,共5页Recent Advances in Ophthalmology

摘　　要：目的研究单酰甘油酰基转移酶2(monoacylglycerol O-acyltransferase 2,MOGAT2)在翼状胬肉组织中的表达及意义。方法采用免疫组织化学染色的方法检测54例翼状胬肉组织标本(原发50例,复发4例)和18例正常结膜组织标本中MOGAT2的表达情况,采用半定量积分法分析免疫组织化学表达的强弱,并对结果进行比较及统计学分析,同时分析翼状胬肉组中MOGAT2的表达与年龄、性别等影响因素及临床病理分期之间的关系。结果免疫组织化学染色结果显示,54例翼状胬肉组织中MOGAT2强阳性表达8例,阳性表达29例,弱阳性表达8例,阴性表达9例;18例正常结膜组织中MOGAT2强阳性表达1例,阳性表达6例,弱阳性表达3例,阴性表达8例。MOGAT2在翼状胬肉组氉和正常结膜组中的表达差异有统计学意义(Z=-2.403,P=0.016)。在翼状胬肉组中,不同年龄、性别MOGAT2的表达差异均无统计学意义(均为P>0.05),MOGAT2的表达强度与文化程度、职业、收入、户外工作时是否防护、是否吸烟、饮酒、血糖、血压情况及初、复发均无显著相关性(均为P>0.05),但与翼状胬肉的临床分期有相关性,进展期翼状胬肉组织中MOGAT2表达显著高于静止期翼状胬肉组织(P<0.05)。结论 MOGAT2在翼状胬肉组织中呈高表达,进展期中的表达强度显著高于静止期其表达水平的升高可能与翼状胬肉的发生发展有显著相关性。Objective To investigate the expression and significance of monoacylglycerol O-acyltransferase 2(MOGAT2)in the tissues of pterygium.Methods Immunohistochemistry staining methods were adopted to detect the expression of MOGAT2 in 54 patients of pterygia(primary in 50 patients and recurrence in 4 patients)and 18 patients of normal conjunctival tissues.The semi-quantitative integration method was used to analyze the strength of immunohistochemical expression,and the results were compared and statistically analyzed,and the relationship between the expression of MOGAT2 in the pterygium group and the factors such as age,sex,and clinical pathological stage was analyzed.Results Immunohistochemical staining showed that,in 54 patients of pterygium,MOGAT2 was strongly expressed in 8 patients,positive expression in 29,weakly positive expression in 8 and negative expression in 9,while in 18 normal conjunctival tissues,strongly positive expression of MOGAT2 was found 1 patient,positive expression in 6,weakly positive expression in 3,negative expression in 8,respectively.Rank-sum test showed that the difference in MOGAT2 expression in the pterygium and normal conjunctiva tissues was statistically significant(Z=-2.403,P=0.016).There was no significant difference in the expression of MOGAT2 between the different age and the sex patients with pterygium(all P>0.05),and there was no significant correlation of MOGAT2 expression with education,occupation,income,protection in outdoor work,smoking,drinking,blood sugar,blood pressure,primary and recurrence pterygium,but significant correlation with the clinical stage of pterygium,that is,MOGAT2 expression in advanced pterygium was overexpressed when compared with tumor tissues in quiescent stage(P<0.05).Conclusion MOGAT2 is highly expressed in pterygium tissues,and the expression in advanced pterygium was significantly upregulated,of which is related to the formation and development of pterygium.

关 键 词：翼状胬肉 MOGAT2 免疫组织化学 

分 类 号：R777.33[医药卫生—眼科]