精液凝固蛋白Ⅰ活性片段的精子抑制活性分析  

Analysis of sperm inhibitory activity of semenogelin Ⅰ active fragment

作　　者：韩飞 孙凯 赵晖[3] Fei Han;Kai Sun;Hui Zhao(Kunming Medical University,Kunming,Yunnan 650500,China;Kunming Second People's Hospital,Kunming,Yunnan 650021,China;First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan 650032,China)

机构地区：[1]昆明医科大学,云南昆明650500 [2]云南省第二人民医院,云南昆明650021 [3]昆明医科大学第一附属医院,云南昆明650032

出　　处：《中国现代医学杂志》2018年第4期26-29,共4页China Journal of Modern Medicine

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金项目(No:2014FB031)

摘　　要：目的制备重组精液凝固蛋白(Sg)及其小分子衍生肽,并分析其对精子活动的抑制作用。方法通过分子克隆及PCR扩增等技术得到实验用重组蛋白,从而对有正常生育能力的男性精子活动进行抑制实验分析。结果在生理条件下,精液Sg的N和C端片段均对精子运动存在抑制作用,其中以N端片段更为显著。结论精液Sg中存在能够抑制精子运动的氨基酸片段,而Sg24~163的作用最为显著。Objectlve To prepare the recombinant semenogelin(Sg)and its different small molecule derived peptides and study the effect of Sg on sperm motility.Methods Molecular cloning and PCR amplification were used to prepare the recombinant Sg,then the recombinant protein was used to test the activity of male sperm with normal reproductive function.Results At the concentration of 200 pg/sperm,the N-terminal fragment of the recombinant Sg had the effect of inhibiting sperm motility(P<0.05),and the C-terminal fragment also inhibited sperm motility(P<0.05).Conclusions There are amino acid fragments in the Sg molecule that can inhibit sperm motility,Sg24-163 has the highest sperm inhibitory activity.

关 键 词：精液凝固蛋白 精子活动 抑制活性 蛋白重组 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]