金银花尺蠖幼虫粪便乙醇提取物体外抗氧化活性  

Antioxidant Activity of Alcohol Extracts of Larva Feces of Honeysuckle Geometrid,Heterolocha jinyinhuaphaga in vitro

作　　者：向玉勇 刘冲 王宗炜 XIANG Yu-yong;LIU Chong;WANG Zhong-wei(School of Biology and Food Engineering,Chuzhou University,Chuzhou 239000,China)

机构地区：[1]滁州学院生物与食品工程学院,滁州239000

出　　处：《天然产物研究与开发》2018年第2期280-285,250,共7页Natural Product Research and Development

基　　金：安徽省高校优秀中青年骨干人才国内外访学研修重点项目(gxfx ZD2016249);安徽省大学生创新创业训练计划(201510377052)

摘　　要：自由基过量累积会影响生物体正常机能,筛选具有自由基清除功能的昆虫代谢产物对于开发虫茶至关重要。为了解金银花尺蠖幼虫粪便提取物的抗氧化活性,在室内测定了金银花尺蠖幼虫粪便乙醇提取物的体外抗氧化活性。结果表明,金银花尺蠖幼虫粪便乙醇提取物具有一定的体外抗氧化活性:对二苯代苦味酰自由基(DPPH)、羟基自由基(·OH)、超氧阴离子自由基(O-·2)、ABTS+·自由基均具有一定的清除能力,在0.05mg/m L时的清除率分别为37.78%、25.31%、21.41%和35.82%;随着提取物浓度的增大,对自由基的清除率逐渐增加,当浓度上升到0.65 mg/m L时的清除率分别为95.52%、69.07%、63.27%和72.46%,分别增加了57.74%、43.76%、41.86%和36.64%,差异达显著水平;对卵黄脂蛋白脂质过氧化也具有一定的抑制作用,在0.05 mg/m L时的抑制率为26.78%,随着提取物浓度的增大,对脂质过氧化的抑制率也逐渐增加,当浓度上升到0.65 mg/m L时的抑制率为50.63%,增加了23.85%,差异达显著水平。在0.05~0.65 mg/m L的浓度范围内,对自由基的清除率、对脂质过氧化的抑制率与浓度之间呈显著的线性关系。综上,金银花尺蠖幼虫粪便提取物具有开发为虫茶的潜力。The excessive accumulation of free radicals would affect the normal function of the organism,hence it is very important for developing sandy tea to select insect metabolites with free radical scavenging function.In order to know the antioxidant activity of extracts from larva feces of Heterolocha jinyinhuaphaga Chu,the antioxidant activity of alcohol extracts from larva feces of H.jinyinhuaphaga in vitro was tested in the laboratory.The results showed that,extracts from larva feces of H.jinyinhuaphaga had a certain clearing effects on DPPH,·OH,O-·2 and ABTS+·.At the concentration of 0.05 mg/mL,its clearing rate on them were 37.78%,25.31%,21.41%and 35.82%separately.With the increasing of concentrations,the clearing rate on them increased,and reached 95.52%,69.07%,63.27%and 72.46%at 0.65 mg/mL,which increased 57.74%,43.76%,41.86%and 36.64%separately,the difference between them reached significant level.The extracts also had a certain prevention effects on peroxidation of yolk lipoprotein with a preventing rate of 26.78%at the concentration of 0.05 mg/mL,it would also increase with the concentrations added,and reached 50.63%at 0.65 mg/mL,which added 23.85%,the difference between them reached significant level.From 0.05 mg/mL to 0.65 mg/mL,the clearing rate on free radicals and prevention rate on peroxidation of yolk lipoprotein all showed a linear connection with the concentrations.Altogether,extracts from larva feces of H.jinyinhuaphaga had the potentiality to be exploited as sandy tea.

关 键 词：金银花尺蠖 粪便提取物 自由基 抗氧化活性 

分 类 号：Q964[生物学—昆虫学]