miR-124对宫颈癌Hela细胞放射敏感性的影响及其机制探讨  

Effect of miR-124 on the radiosensitivity of cervical cancer Hela cel s and its mechanism

作　　者：王梦洁 孟碧[1,2] 高飞 李皓[2] 叶婷[2] 刘阳晨[2] Wang Mengjie;Meng Bi;Gao Fei;Li Hao;Ye Ting;Liu Yangchen(Graduate School of Bengbu Medical College,Anhui Bengbu 233003,China;Taixing People's Hospital,Jiangsu Taixing 225400,China)

机构地区：[1]蚌埠医学院研究生院,安徽蚌埠233003 [2]泰兴市人民医院,江苏泰兴225400

出　　处：《现代肿瘤医学》2018年第6期818-821,共4页Journal of Modern Oncology

基　　金：蚌埠医学院研究生科研创新计划(编号:Byycx1624)

摘　　要：目的:研究miR-124对宫颈癌Hela细胞放射敏感性的影响及其机制。方法:将miR-124模拟物(mimics)、miR-124抑制物(inhibitor)分别转染至Hela细胞中。细胞周期实验检测细胞周期变化。克隆形成实验、细胞凋亡实验研究miR-124对Hela细胞放射敏感性的作用。双荧光素酶报告基因实验、RT-PCR和Western blot检测miR-124的直接靶基因。结果:miR-124模拟物组G0/G1期细胞比例高于对照组,S期细胞比例低于对照组(P均<0.05)。过表达miR-124可降低Hela细胞克隆形成能力,放射增敏比(SER)为1.49。流式细胞凋亡实验证明过表达miR-124可促进放射后细胞凋亡率(P<0.05)。抑制miR-124的表达结果则相反(P<0.05)。双荧光素酶报告基因实验、RT-PCR和Western blot实验证明信号传导与转录激活因子3(signal transducer and activator of transcription 3,STAT3)是miR-124的直接靶基因(P均<0.05)。结论:miR-124可能通过影响STAT3基因的表达来提高宫颈癌Hela细胞的放射敏感性。Objective:To investigate the effect of miR-124 on radiosensitivity of cervical cancer Hela cells and its mechanism.Methods:The miR-124 mimics and miR-124 inhibitor were transfected into Hela cells.Cell cycle experiment showed that miR-124 could influence cell cycle.The important role of miR-124 in radiation sensitivity of Hela cells was confirmed by colony-formation assay and cell apoptosis assay.The target gene of miR-124 was confirmed by luciferase reporter assay,RT-PCR and Western blot.Results:The percentage of G 0/G 1 phase cells in miR-124 mimics group was higher than that in the control group,and the percentage of cells in S phase was lower than that of the control group(all P<0.05).Overexpression of miR-124 could inhibit Hela cells ability of colony formation,and SER was 1.49.Flow cytometric analysis showed that the overexpression of miR-124 had an effect on the proliferation and apoptosis of cervical cancer cells after irradiation(P<0.05).Inhibition of miR-124 would lead to the contrary results(P<0.05).Luciferase reporter gene test,RT-PCR and Western blot experiments showed that STAT3 was a direct target gene of miR-124(all P<0.05).Conclusion:miR-124 may contribute to enhancing radiosensitivity of cervical cancer Hela cells by targeting STAT3.

关 键 词：miR-124 宫颈癌 放射敏感性 

分 类 号：R737.33[医药卫生—肿瘤]