甲基化转移酶基因在乳腺癌组织中的表达及意义  

The expression and significance of DNMTs in breast cancer

作　　者：张丹峰[1] 王曼[1] ZHANG Danfeng;WANG Man(Department of General Surgery,Taihe Hospital,Shiyan 442000,China)

机构地区：[1]湖北省十堰市太和医院普外科,湖北十堰442000

出　　处：《华北理工大学学报（医学版）》2018年第2期89-92,共4页Journal of North China University of Science and Technology：Health Sciences Edition

基　　金：十堰市科技局指导项目(编号:14Y18)

摘　　要：(1)目的检测乳腺癌组织及对应乳腺癌旁组织中甲基化转移酶(DNA methyltransferase,DNMT)的表达情况,分析其表达水平在乳腺癌中的意义。(2)方法应用实时荧光定量PCR(Flurescence quantitative-PCR,FQ-PCR)技术检测53例乳腺癌组织及其中20例对应乳腺癌旁组织中DNMT基因(DNMT1、DNMT2、DNMT3a、DNMT3b)mRNA表达水平,并分析其表达与乳腺癌临床病理特征之间关系。(3)结果乳腺癌组织中DNMT1、DNMT2、DNMT3a、DNMT3b的mRNA相对表达水平均高于对应癌旁组织,差异有统计学意义(P<0.05);且DNMT1、DNMT3a、DNMT3bmRNA的表达水平在是否淋巴转移之间的差异具有统计学意义(P<0.05),而在乳腺癌其他临床病理特征参数之间差异无统计学意义(P>0.05)。(4)结论乳腺癌组织中DNMT1、DNMT2、DNMT3a、DNMT3b的基因表达水平明显高于对应的癌旁组织;不同DNMT基因表达水平的检测可以为了解乳腺癌的发病机理提供参考依据;高表达的DNMT可能成为乳腺癌预后的指标之一。Objective To detect the expression and clinical significances of DNMT mRNA in breast cancer cases and adjacent tissues.Methods FQ-PCR method was used to detecte the expression levels of DNMT1、DNMT2、DNMT3a、DNMT3b mRNA and analyzed their relationship in 53 breast cancer and 20 adjacent tissues.Results The expression level of DNMT1、DNMT3a、DNMT3b mRNA were higher than those in adjacent tissues(P<0.05).The expression of DNMT1、DNMT3a、DNMT3b mRNA were correlated with lymph node metastasis and were not correlated with other clinicopathological features.Conclusion The gene expression level of DNMT1,DNMT3a and DNMT3b in breast cancer is significantly higher than that of the adjacent tissues;The detection of different DNMT gene expression levels provide a reference for the pathogenesis of breast cancer;Higher expression of DNMTs may be a biomarker for the prognosis of breast cancer.

关 键 词：乳腺癌 癌旁组织 甲基化转移酶 

分 类 号：R737.9[医药卫生—肿瘤]