榨菜根肿病生防细菌的筛选、鉴定及评价  

Screening,identification and evaluation of antagonistic bacteria against clubroot of mustard

作　　者：蒋欢 彭玉梅 闫玉芳 张勇 吴朝君 何本洪 王旭祎 JIANG Huan;PENG Yumei;YAN Yufang;ZHANG Yong;WU Chaojun;HE Benhong;WANG Xuyi(Southeast Chongqing Academy of Agricultural Sciences,Fuling　408099,China)

机构地区：[1]重庆市渝东南农业科学院,涪陵408099

出　　处：《植物保护》2018年第2期104-110,共7页Plant Protection

基　　金：涪陵区应用技术研究与开发资金(FLKJ;2014ABB2067;FLKJ;2015ABB1050);重庆市社会事业与民生保障科技创新专项(cstc2015shms-ztzx0129)

摘　　要：近年来,由于十字花科根肿病在榨菜上扩展蔓延,严重影响其产量,造成巨大的经济损失。本研究筛选生防菌并研究其对榨菜根肿病的控制作用,为其生物防治应用奠定基础。研究发现茎瘤芥等十字花科蔬菜根肿病菌根经过腐烂处理后,休眠孢子的萌发率可到达70%以上。故本文采用稀释法从榨菜根肿病菌根中分离筛选出一株可刺激休眠孢子萌发的菌株PB19。并测定了PB19与根肿病菌互作的最适温度和最适pH,温室盆栽试验和大田小区试验测定菌株PB19对榨菜根肿病的防治效果。最后将纯化后的菌株送至中国食品发酵工业研究院微生物检测中心(FMIC)进行种属鉴定。结果表明:PB19与根肿菌互作的最适温度为20~25℃,最适pH为5.5~7.0。温室盆栽结果显示PB19对榨菜根肿病的防治效果为71.96%。而大田试验结果显示PB19对榨菜根肿病的防治效果为18.04%。FMIC的鉴定结果显示PB19为假单胞菌属Pseudomonas sp.。In recent years,due to the spread of the Cruciferae clubroot in mustard,it has seriously affected its production and caused great economic loss.Biocontrol bacteria has been screened and studied for control of mustard clubroot,laying a foundation for its application in biological control.Some studies have found that the germination rate of the dormant spores could reach more than 70%after the decay process of the root of cruciferous vegetables.Here the strain PB19 was isolated from the mustard clubroot,which could stimulate the germination of resting spores by dilution method.First,the optimum temperature and pH value for the interaction between PB19 and Plasmodiophora brassicae were measured.The control efficiency of PB19 against clubroot of mustard was evaluated in pot experiments and field plot experiments.The strain was identified by Microbiology Identification Center of CNRIFFI(FMIC).The test results showed that the optimum temperature for the interaction between PB19 and P.brassicae was 20-25℃,and the suitable pH value was 5.5-7.0.The control efficiency of PB19 against clubroot of mustard was 71.96%in pot experiments,and only 18.04%in field plot experiments.PB19 was identified as Pseudomonas sp.by FMIC.

关 键 词：榨菜 根肿病 生防细菌 假单胞菌 生物防治 

分 类 号：S436.37[农业科学—农业昆虫与害虫防治]