多发性骨髓瘤和慢性淋巴细胞白血病患者血浆lncRNA MALAT1、LincRNA-p21和GAS5的表达水平及其诊断意义  

Expression levels of plasma lncRNA MALAT1,LincRNA-p21 and GAS5 in patients with multiple myeloma and chronic lymphocytic leukemia and their diagnostic significances

作　　者：黄琦 沙辉[1] 陈龙 吕龙龙 徐声鸣 张泽天[1] 赵松[1] 靳凤艳[2] 牛丰[1] HUANG Qi;SHA Hui;CHEN Long;LYU Longlong;XU Shengming;ZHANG Zetian;ZHAO Song;JIN Fengyan;NIU Feng(Department of Spine Surgery,First Hospital,Jilin University,Changchun 130021,China;Cancer Center,First Hospital,Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学第一医院脊柱外科,吉林长春130021 [2]吉林大学第一医院肿瘤中心,吉林长春130021

出　　处：《吉林大学学报（医学版）》2018年第2期339-345,共7页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81670190)

摘　　要：目的:探讨多发性骨髓瘤(MM)和慢性淋巴细胞白血病(CLL)患者血浆长链非编码RNA(lncRNA)MALAT1、LincRNA-p21和GAS5相对表达水平的差异,阐明3种lncRNA在MM与CLL鉴别诊断中的意义。方法:选取60例MM患者(MM组)、60例CLL患者(CLL组)和60名健康体检者(对照组)作为研究对象,对3组研究对象血浆lncRNA MALAT1、LincRNA-p21和GAS5的表达水平进行检测和比较。结果:MM组患者血浆lncRNA MALAT1和GAS5相对表达水平明显高于其他2组(P<0.05)。CLL组患者血浆LincRNA-p21相对表达水平明显低于其他2组(P<0.05)。血浆LincRNA-p21诊断CLL的受试者工作特征曲线(ROC)下面积(AUC)为0.850,95%可信区间(CI)为0.780~0.921;血浆lncRNA MALAT1和GAS5在MM诊断中的AUC分别为0.898和0.815,95%CI分别为0.836~0.959和0.740~0.890;血浆lncRNA MALAT1、LincRNA-p21和GAS5在CLL和MM鉴别诊断中的AUC分别为0.878、0.778和0.805,95%CI分别为0.814~0.942、0.691~0.865和0.727~0.882。结论:血浆lncRNA MALAT1和GAS5的高表达与MM的发病有关,而血浆LincRNA-p21低表达与CLL的发病有关,上述3种lncRNA的血浆相对表达水平可作为MM和CLL诊断中的辅助指标。Objective:To explore the differences of the relative expression levels of plasma long chain non encoding RNA(lncRNA)MALAT1,LincRNA-p21 and GAS5 in the patients with multiple myeloma(MM)and chronic lymphocytic leukemia(CLL),and to clarify their significances in the differential diagnosis of MM and CLL.Methods:A total of 60 cases of MM patients(MM group),60 cases of CLL patients(CLL roup)and 60 healthy persons after physical examinations(control group)were selected as the subjects.The plasma levels of lncRNA MALAT,LincRNA-p21 and GAS5 of the subjects in three groups were detected and compared.Results:The relative expression levels of lncRNA MALAT1 and GAS5 in plasma of the patients in MM group were significantly higher than those in the other two groups(P<0.05);the relative expression level of LincRNA-p21 in plasma of the patients in CLL group was significantly lower than those in the other two groups(P<0.05).The area under receiver operating characteristic curve(ROC)(AUC)of plasma LincRNA-p21 in the diagnosis of CLL was 0.850,its 95%confidence interval(CI)was 0.780-0.921.The AUC of plasma lncRNA MALAT1 and GAS5 in the diagnosis of MM were 0.898 and 0.815;their 95%CI were 0.836-0.959 and 0.740-0.890,respectively.The AUC of plasma lncRNA MALAT1,LincRNA-p21 and GAS5 in the differential diagnosis of CLL and MM were 0.878,0.778 and 0.805,and their 95%CI were 0.814-0.942,0.691-0.865 and 0.727-0.882,respectively.Conclusion:The incidence of MM is related with the high expressions of lncRNA MALAT1 and GAS5 in plasma and the incidence of CLL is related with the low expression of LincRNA-p21 in plasma.The relative expression levels of the above lncRNA can be used as the auxiliary indexes in the diagnosis of MM and CLL.

关 键 词：多发性骨髓瘤 慢性淋巴细胞白血病 血浆 长链非编码RNA 

分 类 号：R733.3[医药卫生—肿瘤]