蚕豆保健品中左旋多巴的测定方法研究  

Study on the determination of L-Dopa in faba bean dietary supplement

作　　者：赵爱平[1,2] 冯德建 许洋[1,2] 邹燕 张琦[3] 李怀平 ZHAO Aiping;FENG Dejian;XU Yang;ZOU Yan;ZHANG Qi;LI Huaiping(National Institute of Measurement and Testing Technology,Chengdu 610021,China;Standard and Testing Technology of Tea Key Laboratory of Sichuan Province,Chengdu 610021,China;Sichuan Institute for Food and Drug Control,Chengdu 611731,China)

机构地区：[1]中国测试技术研究院,四川成都610021 [2]茶叶标准与检测技术四川省重点实验室,四川成都610021 [3]四川省食品药品检验检测院,四川成都611731

出　　处：《中国测试》2018年第3期57-60,共4页China Measurement & Test

基　　金：国家重点研发计划项目(2016YFF0202300)

摘　　要：建立蚕豆保健品中左旋多巴含量的高效液相色谱测定方法。样品经0.10 mol/L盐酸溶液超声提取20 min,XDB C18色谱柱分离,以甲醇-10 mmol/L磷酸二氢钾溶液(体积比2∶98)等度洗脱,280 nm下检测,外标法定量。结果表明:左旋多巴质量浓度在1.656~265.0 mg/L范围内与峰面积呈现良好的线性关系,方法的平均加标回收率为98.4%~100.9%,相对标准偏差为0.83%~2.18%,检出限为1.9 mg/kg,定量限为6.3 mg/kg。能够满足蚕豆保健品中左旋多巴含量的检测和质量控制需要。A method was developed for the determination of L-Dopa in faba bean dietary supplement by HPLC.After extracted by Ultrasonic-assisted with 0.10 mol/L hydrochloric acid solution for 20 minutes,the sample was separated on a XDB C18 column with the isocratic elution of methanol and 10 mmol/L potassium phosphate monobasic solution(2:98,V/V)as mobile phase.The detection wavelength was 280 nm and the sample was quantified by the external standard method.The results showed that a good linearity was obtained for L-Dopa concentration in the range of 1.656-265.0 mg/L,the average recoveries of L-Dopa ranged from 98.4%to 100.9%and the RSDs were 0.83%-2.18%.The detection limit and quantitation limit of L-Dopa were 1.9 mg/kg and 6.3 mg/kg,respectively.The method is suitable for the determination and quality control of L-Dopa in faba bean dietary supplement.

关 键 词：蚕豆保健品 左旋多巴 高效液相色谱仪 蚕豆粉 蚕豆茶 

分 类 号：O657.72[理学—分析化学]