曲古抑菌素A对青光眼滤过术术后滤过道瘢痕化细胞模型Ⅰ型胶原蛋白表达的影响  

Effect of TSA on the expression of type Ⅰ Collagen in the scarring cytology model after glaucoma filtering surgery

作　　者：李晓艳[1] 李蓉[1] 周凌霄[1] 邓颖[1] LI Xiao-yan;LI Rong;ZHOU Ling-xiao;DENG Ying;LI Rong;DENG Ying(Department of Ophthalmology,The First Affiliated Hospital of Xi′an Medical College,Xi′an Shaanxi 710077,China)

机构地区：[1]西安医学院第一附属医院眼科,陕西西安710077

出　　处：《蚌埠医学院学报》2017年第12期1596-1598,共3页Journal of Bengbu Medical College

基　　金：陕西省教育厅专项科研计划项目(2013JK0785)

摘　　要：目的:研究曲古抑菌素A(TSA)对青光眼滤过术(GFS)术后滤过道瘢痕化细胞模型Ⅰ型胶原蛋白表达的影响。方法:构建GFS术后滤过道瘢痕化细胞模型,RT-PCR、Western blotting法分别检测600 nmol/L TSA对组蛋白乙酰基转移酶Ⅰ型胶原蛋白mRNA及蛋白表达的影响。结果:RT-PCR、Western blotting均证实600 nmol/L TSA对Ⅰ型胶原蛋白在基因转录及蛋白表达水平有抑制作用(P<0.01和P<0.05)。结论:TSA能减弱GFS术后滤过道瘢痕化细胞模型中Ⅰ型胶原纤维基因转录及蛋白表达水平。Objective:To investigate the effects of trichostatin A(TSA)on the expression of typeⅠCollagen in the scarring cytology model after glaucoma filtering surgery(GFS).Methods:The cytology model of scarring after GFS was established.The effects of 600 nmol/L TSA on the expression levels of typeⅠCollagen mRNA and protein were detected using RT-PCR and Western blotting,respectively.Results:The results of RT-PCR and Western blotting showed that the 600 nmol/L TSA could inhibit the expression levels of typeⅠCollagen mRNA and protein(P<0.01 and P<0.05).Conclusions:TSA can reduce the expression levels of typeⅠCollagen mRNA and protein in the scarring cytology model after GFS.

关 键 词：青光眼 曲古抑菌素A Ⅰ型胶原纤维 成纤维细胞 

分 类 号：R775[医药卫生—眼科]