HSP90在胆管癌组织与细胞中的表达及17-AAG对胆管癌细胞株的影响  

Expression of HSP90 in cholangiocarcinoma tissues and cells and the effect of 17-AAG on cholangiocarcinoma cell line

作　　者：张晶锐 高峰 李荣江 邱振雄 黄振华 ZHANG Jingrui;GAO Feng;Li Rongjiang;Qiu Zhexiong;HUANG Zhenhua(General Surgery of Shenzhen Baoan District Central Hospital,Shenzhen 518102,China;General Surgery of Southern Medical University Shenzhen Hospital,Shenzhen 518100,China)

机构地区：[1]深圳市宝安区中心医院普通外科,广东深圳518102 [2]南方医科大学深圳医院普通外科,广东深圳518100

出　　处：《中国现代普通外科进展》2017年第12期933-936,共4页Chinese Journal of Current Advances in General Surgery

基　　金：深圳市科技研发资金项目(JCYJ20150403150555636)

摘　　要：目的 :探讨HSP90在胆管癌组织与细胞中的表达情况及17-AAG对胆管癌TE-1细胞株的影响。方法:选择我院2015年7月—2016年7月收治的40例胆管癌患者作为研究对象,分别检测患者胆管癌组织、癌旁组织及正常组织的HSP90α表达情况,应用不同浓度的17-AAG对胆管癌TE-1细胞株进行处理并检测HSP90及其效应蛋白HIF-1α的表达情况。结果:胆管癌组织的总阳性率为82.5%,高于癌旁组织的35.0%与正常组织的20.0%(均P<0.05)。胆管癌组织的强阳性表达率为37.5%,高于癌旁组织的10.0%与正常组织的5.0%(均P<0.05)。患者胆管癌组织的HSP90α表达与浸润深度、淋巴结转移无关(P>0.05),但与TNM分期及肿瘤直径有明显相关性(P<0.05)。随着17-AAG处理浓度的升高,HSP90α/β-actin、HIF-1α/β-actin降低,不同处理浓度下胆管癌TE-1细胞株HSP90α及其效应蛋白HIF-1α表达均有统计学意义(P<0.05)。结论:HSP90在胆管癌组织及细胞中高表达,且表达情况与临床分期及肿瘤直径有关。17-AAG可下调胆管癌TE-1细胞株中HSP90α及HIF-1α表达,对于临床治疗有一定指导作用。Objective:To investigate the expression of HSP90 in cholangiocarcinoma tissues and cells,and the effect of 17-AAG on cholangiocarcinoma cell line.Methods:Forty patients with cholangiocarcinoma admitted to our hospital from July 2015 to July 2016 were selected as study subjects.Expression of HSP90 in cholangiocarcinoma tissues,paracancerous tissues and normal tissues was detected respectively.Cholangiocarcinoma cell lines were treated with different concentrations of 17-AAG,and expression of HSP90 and its effect protein HIF-1αwas detected.Results:The total positive rate in cholangiocarcinoma tissues(82.5%)was higher than paracancerous tissues(35.0%)and normal tissues(20.0%)(all P<0.05).The strong positive expression rate in cholangiocarcinoma tissues(37.5%)was higher than paracancerous tissues(10.0%)and normal tissues(5.0%)(all P<0.05).There was no significant correlation between expression of HSP90 in cholangiocarcinoma tissues and invasion depth,lymph node metastasis(P>0.05),but there was a significant correlation with TNM stage and tumor diameter(P<0.05).With the increase of treatment concentration of 17-AAG,HSP90α/β-actin and HIF-1α/β-actin decreased,and there were significant differences in expression of HSP90αand its effect protein HIF-1αin cholangiocarcinoma cell lines under different treatment concentrations(P<0.05).Conclusion:HSP90 is highly expressed in cholangiocarcinoma tissues and cells and its expression is related to clinical stage and tumor diameter.17-AAG can down regulates the expression of HSP90αand HIF-1αin TE-1 cells,which plays a certain guiding role in clinical treatment.

关 键 词：胆管癌 HSP90 17-AAG TE-1细胞株 

分 类 号：R575.7[医药卫生—消化系统]