骨形态发生蛋白2联合基因转染骨髓间充质干细胞复合同种异体骨修复极限骨缺损  

Bone defect repair using allogenic bone carrying bone morphogenetic protein-2 and gene transfected bone marrow mesenchymal stem cells

作　　者：王小志 杨桦 张辰 何惠宇 巴娇娇 Wang Xiao-zhi;Yang Hua;Zhang Chen;He Hui-yu;Ba Jiao-jiao(Department of Stomatology,People’s Hospital of Changji Hui Autonomous Prefecture,Changji 831100,Xinjiang Uygur Autonomous Region,China;Infirmary of the Xinjiang Branch of China Southern Airlines,Urumqi 830002,Xinjiang Uygur Autonomous Region,China;Department of Stomatology,General Hospital of Xinjiang Construction Corps,Urumqi 830000,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆昌吉回族自治州人民医院口腔科,新疆维吾尔自治区昌吉市831100 [2]中国南方航空股份有限公司新疆分公司医务室,新疆维吾尔自治区乌鲁木齐市830002 [3]新疆生产建设兵团总医院口腔科,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《中国组织工程研究》2018年第9期1313-1318,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81060088)：三维打印构建组织工程化牙槽骨的实验研究

摘　　要：背景:组织工程技术为骨缺损带来新的治疗路径,生物基因转染优化了组织工程骨修复骨缺损的效果。目的:观察骨形态发生蛋白2与碱性成纤维细胞生长因子基因转染骨髓间充质干细胞/同种异体骨修复绵羊极限骨缺损的效果。方法:取9只阿勒泰大尾绵羊,制作双侧髂骨极限性骨缺损模型,左侧植入骨形态发生蛋白2与碱性成纤维细胞生长因子基因转染骨髓间充质干细胞/同种异体骨复合材料(实验组),其中3只右侧植入骨髓间充质干细胞/同种异体支架骨复合材料(细胞支架组),3只右侧植入同种异体支架骨材料(同种异体骨组),3只右侧植入β-磷酸三钙材料(磷酸三钙组)。植入后4,8,12周进行组织学、免疫组织化学染色观察。结果与结论:(1)组织学观察:植入12周时,实验组同种异体骨材料大范围吸收,破骨样细胞逐渐减少,成骨样细胞增多,骨小梁周围骨纤维基质逐渐致密,可见大面积成片状新骨,骨小梁结构清晰,骨改建明显,术区始终未见炎性细胞浸润;其余3组支架骨材料降解,但无明显骨改建;(2)免疫组织化学观察:植入12周时,实验组骨涎蛋白与Ⅰ型胶原呈强阳性表达,其余3组骨涎蛋白与Ⅰ型胶原呈弱表达;(3)结果表明:骨形态发生蛋白2联合碱性成纤维细胞生长因子基因转染的骨髓间充质干细胞复合同种异体骨,可完全修复羊极限骨缺损。BACKGROUND:Bone tissue engineering technology brings a new path to treat bone defects,and moreover,gene transfection optimizes the effects of tissue-engineered bone in bone repair.OBJECTIVE:To observe the effect of allogenic bone carrying bone morphogenetic protein-2(BMP-2)and basic fibroblast growth factor(bFGF)transfected bone marrow mesenchymal stem cells(BMSCs)in the repair of critical-sized bone defects of the sheep ilium.METHODS:Nine Altay sheep were taken to make animal models of critical-sized bone defects in the bilateral ilia.Allogenic bone carrying BMP-2 and bFGF-transfected BMCSCs was implanted into the left side of the ilium(n=9,experimental group),while allogenic bone carrying BMSCs(n=3,cell scaffold group),allogenic bone(n=3,allogenic bone group)andβ-tricalcium phosphate(n=3,β-TCP group)were implanted into the right side of the ilium,respectively.Histological observation and immunohistochemical staining were performed at 4,8,12 weeks after implantation.RESULTS AND CONCLUSION:Findings from histological observation showed that:at 12 weeks after implantation,the experimental group exhibited obvious bone remodeling,largely absorbed bone allograft,gradually reduced osteoclast-like cells and increased osteoblast-like cells,clear trabecular structure,dense bone matrix around the trabecular bone,and large area of flaky new bone tissues.Bone materials also degraded in the other three groups,but no remodeling occurred.Immunohistochemical staining showed that at 12 weeks after implantation,bone sialoprotein type I collagen in the experimental group were strongly positive,while they were weakly expressed in the remaining three groups.Overall,our findings indicate that the allogenic bone carrying BMP-2 and bFGF-transfected BMSCs can thoroughly repair critical-sized bone defects of the sheep ilium.

关 键 词：骨形态发生蛋白质类 成纤维细胞生长因子2 干细胞 组织工程 

分 类 号：R394.2[医药卫生—医学遗传学]