大鼠喉返神经挤压伤后喉肌及神经变化的初步研究  

Preliminary Research in Response to Recurrent Laryngeal Nerve Crush Injury of Rats

作　　者：吴涛[1] 沈叶[1] 王文杰[1] 黄忠明[1] 王时光[1] 瞿军[1] WU Tao;SHEN Ye;WANG Wen-jie;HUANG Zhong-ming;WANG Shi-guang;QU Jun(Department of General Surgery,Fengxian District Central Hospital,Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus,Shanghai 201499,China)

机构地区：[1]上海市奉贤区中心医院(上海交通大学附属第六人民医院南院)普外科,上海201499

出　　处：《神经损伤与功能重建》2018年第3期127-129,152,共4页Neural Injury and Functional Reconstruction

摘　　要：目的:评估大鼠喉返神经挤压伤后的自我修复效果。方法:大鼠30只,随机分为对照组和实验组各15只,实验组行右侧喉返神经5 mm长度的挤压损伤,对照组仅暴露神经,不造成损伤。在术后4周、8周及12周检测各组动物的一般状况、发声功能、声带运动、受损神经组织学及甲杓肌中脑源性神经营养因子(BDNF)的表达情况。结果:术后随着时间的延长,实验组的发声功能、声带运动及受损神经的轴突个数逐渐恢复,直到术后12周,上述指标恢复至正常水平。术后4周,右侧甲杓肌中BDNF的表达量最低,之后逐渐增加,直到术后12周BDNF的表达量远远超过对照组水平(P<0.05)。结论:单侧喉返神经挤压伤后存在自我修复,该修复能力可能与神经营养因子的表达有关。Objective:To evaluate the restorative effect of spontaneous reinnervation in rats following recurrent laryngeal nerve(RLN)crush injury.Methods:Total 30 male rats were randomly divided into control or experimental groups,with 15 rats per group.Experimental group underwent right recurrent laryngeal nerve crush injury on a 5 mm segment.Control group was subject to nerve exposure but no nerve injury.General conditions,vocalization,vocal fold movements,nerve histology,and expression of the brain-derived neurotrophic factor(BDNF)in thyroarytenoid muscle were evaluated at different time points 4,8,and 12 weeks post-procedure.Results:Vocalization,vocal fold movements,and number of axons in experimental group rats were gradually restored with time and reached normal levels at week 12.Expression level of BDNF in the right thyroarytenoid muscle reached a minimum at week 4,then gradually increased until expression level far surpassed that of the control group at week 12(P<0.05).Conclusion:Spontaneous reinnervation was present after RLN crush injury,and this ability may be closely related to the expression of neurotrophic factors.

关 键 词：喉返神经 神经修复 声带麻痹 脑源性神经营养因子 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]