非小细胞肺癌移植瘤侵袭组织中血清蛋白的分布  

Immunohistochemical analysis of serum proteins in xenografted non-small cell lung cancer tissues in nude mice

作　　者：白玉勤 吴宝 张龙 刘晓辉[1,2] 其木格 刘帅莹[1,2] 韦学磊 王徽[5] BAI Yuqin;WU Bao;ZHANG Long;LIU Xiaohui;QI Muge;LIU Shuaiying;WEI Xuelei;WANG Hui(Department of Pathology,Medical College of Chifeng University,Chifeng 024000;Department of Pathology,Affiliated Hospital of Chifeng University,Chifeng 024005;Department of Histology and Embryology,Medical College of Chifeng University,Chifeng 024000;Department of Medical Examination,Affiliated Hospital of Chifeng University,Chifeng 024005;Department of Neurology,Affiliated Hospital of Chifeng University, Chifeng 024005,Inner Mongolia,China)

机构地区：[1]赤峰学院医学院病理学教研室,内蒙古赤峰024000 [2]赤峰学院附属医院病理科,内蒙古赤峰024005 [3]赤峰学院医学院组织胚胎学教研室,内蒙古赤峰024000 [4]赤峰学院附属医院输血科,内蒙古赤峰024005 [5]赤峰学院附属医院神经内科,内蒙古赤峰024005

出　　处：《癌变．畸变．突变》2018年第2期132-135,139,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：内蒙古自然科学基金(2013MS1113)

摘　　要：目的:局部侵袭是肺癌患者死亡的主要原因之一,本研究探讨肺癌侵袭组织中血清蛋白的分布及其与血管内皮生长因子(VEGF)和表皮生长因子受体(EGFR)蛋白的关系。方法:建立非小细胞肺癌(NSCLC)A549细胞注射裸鼠背部皮下的异种移植模型,利用活体冷冻技术(IVCT)制备标本,免疫组化分析Albumin、Ig G、Ig M、VEGF、EGFR蛋白分布。结果:观察到NSCLC侵袭组织周围结缔组织、血管、间质及细胞外基质有Albumin和Ig G1、Ig M主要在侵袭组织周围结缔组织和血管内,癌细胞外基质未观察到Ig M。肺癌细胞浆内VEGF蛋白呈细颗粒状,但是癌细胞浆和膜未观察到EGFR蛋白分布。结论:利用IVCT技术能清晰观察到侵袭组织周围结缔组织、血管和细胞外基质中不同分子量血清蛋白分布,NSCLC侵袭组织细胞外基质Albumin、Ig G1和Ig M免疫组织化学染色分布主要依据其分子量,与VEGF和EGFR蛋白分布无关。OBJECTIVE:Local invasion is a leading cause of death in patients with lung cancer.To date,there has been no report on distribution of VEGF,EGFR and serum protein in invasive tissues of lung cancer using in vivo cryotechnique(IVCT)method.METHODS:The human A549 cell line from a non-small cell lung cancer(NSCLC)was used and cells were subcutaneously injected to the dorsal flank of nude mice.In vivo tumor samples were prepared by IVCT method.At the same time,protein distribution of albumin,IgG,IgM,VEGF and EGFR were examined by immunohistochemistry method.RESULTS:The distribution of albumin and IgG in connective tissues around the tumor mass,blood vessels,interstitium and extracellular matrix of NSCLC's invasive parts can be clearly observed by using the IVCT method.IgMs were mainly located in connective tissues around the tumor mass and blood vessels,but their distribution could not be observed in extracellular matrix of cancer cells.VEGF protein was located in cytoplasm of lung cancer cells,and it had fine granular morphology,but there was no distribution of EGFR in cytoplasm or membrane of cancer cells.CONCLUSION:The immunodistribution of different molecular weight of serum proteins in connective tissues around the tumor mass,blood vessels,interstitium and extracellular matrix can be clearly observed.The permeability of NSCLC's invasive part for albumin,IgG1 and IgM in extracellular matrix mainly depended on their respective molecular weights,but not on the protein distribution of VEGF and EGFR.

关 键 词：活体冷冻技术 NSCLC 血清蛋白 免疫组化分析 

分 类 号：R361.2[医药卫生—病理学]