表面增强拉曼光谱法快速检测饮料中玫瑰红B  

Rapidly Screening Rhodamine B in Drinks by Surface Enhanced Raman Spectroscopy

作　　者：王乐[1] 张爱霞[1] 陈晞[1] 郑新华[1] 何桂华[1] WANG Le;ZHANG Ai-xia;CHEN Xi;ZHENG Xin-hua;HE Gui-hua(Jinan Entry-Exit Inspection and Quarantine Bureau,Jinan 250014,Shandong,China)

机构地区：[1]济南出入境检验检疫局,山东济南250014

出　　处：《食品研究与开发》2018年第7期126-130,共5页Food Research and Development

基　　金：国家质检总局科研项目(2015IK212;2017IK039);山东出入境检验检疫局科技项目(SK201616)

摘　　要：采用多孔银丝表面增强拉曼(surface enhanced Raman scattering,SERS)基底,建立快速检测饮料中人工合成着色剂玫瑰红B的分析方法。饮料样品无需前处理,滤膜过滤后采用循环伏安法扫速25 m V/s制备的多孔银丝基底静置萃取8 min后即进行拉曼测试,结合固体标准品拉曼谱图和密度泛函理论(density functional theory,DFT)计算结果对玫瑰红B表面增强拉曼谱图中的特征峰进行了归属,以对玫瑰红B进行定性和定量测定。结果表明,1 277 cm^(-1)处的峰强度与玫瑰红B浓度的线性范围为5×10^(-8)~2.5×10^(-6) mol/L,检出限为5×10^(-9) mol/L。该方法原位萃取、测试时间只需9 min,可应用于饮料样品中玫瑰红B的快速检测,回收率在90.4%~92.3%,相对标准偏差小于5.2%。Rapidly screening method of man-made synthetic colorant Rhodamine B in food was established based on porous Ag fiber as surface enhanced Raman scattering(SERS)substrate.Without any sample pretreatment,silver fiber substrate synthesized with electrochemical assistance by Cyclic Voltammetry(CV)scanning at 25 mV/s was immersed in the membrane filtrated drink samples for 8 minutes without stirring.After that,the silver substrate was directly introduced for Raman analysis.The characteristic SERS spectrum peaks of Rhodamine B were attributed together with standard Raman and density functional theory(DFT)Raman spectra,followed by qualitative and quantitative analysis.The band at 1 277 cm-1 was selected as the characteristic peak for quantitative determination,showing good linearity from 5伊10-8 to 2.5伊10-6 mol/L and detection limit of 5伊10-9 mol/L.It only takes 9 minutes for the in-situ extraction coupled with Raman test of the proposed method,which was successfully applied to fast identify of Rhodamine B in drink samples with recovery in the range of 90.4%-92.3%and good relative standard deviations(RSDs)less than 5.2%.

关 键 词：多孔银丝基底 表面增强拉曼光谱 玫瑰红B 定性 定量 

分 类 号：O657.37[理学—分析化学]