小鼠角膜碱烧伤后缺氧诱导因子-1α和水通道蛋白-1在角膜中的表达  

Expression of hypoxia inducible factor-1α and aquaporin-1 in the cornea after alkali burn

作　　者：李康 安文珍 杜少博[1] 张颖[1] 张璇 高岚[1] LI Kang;AN Wen-Zhen;DU Shao-Bo;ZHANG Ying;ZHANG Xuan;GAO Lan

机构地区：[1]兰州大学生命科学学院,甘肃省兰州市730000

出　　处：《眼科新进展》2018年第4期306-309,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:31471953);甘肃省自然科学基金资助(编号:17JR5RA220)~~

摘　　要：目的检测小鼠角膜碱烧伤后缺氧诱导因子1α(hypoxia inducible factor-4 alpha,HIF-1α)及水通道蛋白-1(aquaporin-1,AQP1)的表达情况,探讨角膜碱烧伤后HIF-1α及AQP1在其损伤机制中的作用。方法选用健康成年雌性昆明系小鼠48只,左眼为对照组,右眼用1 mol·L^(-1)NaOH建立角膜碱烧伤模型,并随机分为碱烧伤后1 d组、4 d组、7 d组、14 d组,每组12只,免疫荧光染色法和实时荧光定量PCR法检测碱烧伤后角膜HIF-1α、AQP1的表达情况。结果免疫荧光染色结果显示,对照组中HIF-1α在角膜上皮基底膜弱表达,碱烧伤后1 d HIF-1α在角膜上皮层表达开始增强,4 d在角膜上皮层和基质层均表达,至7 d时HIF-1α表达达到峰值;对照组中AQP1在角膜内皮层弱表达,碱烧伤后1 d,AQP1在角膜内皮层表达开始增强,4 d和7 d时AQP1在角膜内皮层和基质层均表达,且表达更强。实时荧光定量PCR结果显示,与对照组(188.70±33.99)相比,碱烧伤后1 d组(269.70±15.68)、4 d组(350.50±67.26)、7 d组(272.10±6.88)的HIF-1αmRNA相对表达量增加,差异均有统计学意义(均为P<0.05);与对照组(36.43±3.95)相比,碱烧伤后1 d组(61.90±5.45)、7 d组(48.34±1.33)的AQP1 mRNA相对表达量增加,差异均有统计学意义(均为P<0.05)。结论碱烧伤引起了角膜病理性变化,HIF-1α和AQP1参与碱烧伤后角膜损伤的病理机制。Objective To detect the expressions of hypoxia inducible factor-1α(HIF-1α)and aquaporin-1(AQP1)in the alkali-burn cornea and investigate the roles of HIF-1αand AQP1 in alkali-burn mechanisms.Methods Totally 48 healthy adult female Kunming mice were selected,and the left eyes were treated with saline as the control group,and the model of corneal alkali burn was established with concentration of 1 mol·L-1 NaOH on the right eyes,and then these rats were randomly divided into 1-day,4-day,7-day and 14-day group.The expressions of HIF-1αand AQP1 in the cornea were detected using immunofluorescence and qRT-PCR after corneal alkali burn model was successful.Results In the control group,HIF-1αwas expressed in the corneal epithelial basement membrane,but it was increased in the corneal epithelium layer 1 day after alkali burn,and it was expressed in the corneal epithelium layer and stroma 4 days,and peaked 7 days after alkali burn;AQP1 was weakly expressed only in the endothelial layer in the control group,but it was increased in the corneal endothelial layer 1 day after alkali burn,and it was strongly expressed in the endothelial cell layer and the stroma 4 days and 7 days after alkali burn.qRT-PCR indicated that the relative expression level of HIF-1αmRNA was 269.70±15.68 in 1-day group,350.50±67.26 in 4-day group and 272.10±6.88 in 7-day group,respectively,which all were higher than that in the control group(188.70±33.99),with significant difference(P<0.05).In addition,the relative expression level of AQP1 mRNA was 61.90±5.45 in 1-day group and 48.34±1.33 in 7-day group,which were significantly higher than that in the control group(36.43±3.95),with statistical difference(P<0.05).Conclusion Alkali burn can cause the pathological changes in the cornea,and HIF-1αand AQP1 involve in corneal injury after alkali burn.

关 键 词：角膜 碱烧伤 缺氧诱导因子-1Α 水通道蛋白-1 

分 类 号：R772.2[医药卫生—眼科]