沙门氏菌ATCC 13311脂多糖及类脂A的提取及纯化  

Extraction and Purification of Lipopolysaccharide and Lipid A fromSalmonella ATCC 13311

作　　者：薛丽芝 XUE Lizhi(Food Science Department,Shanghai Food Science and Technology School,Shanghai 201599,China)

机构地区：[1]上海食品科技学校,上海201599

出　　处：《常州大学学报（自然科学版）》2018年第2期87-92,共6页Journal of Changzhou University：Natural Science Edition

摘　　要：沙门氏菌ATCC 13311作为一种革兰氏阴性菌,是引起人畜肠道传染病的病原体,严重威胁公众健康并影响食品环境安全。脂多糖(LPS)是革兰氏阴性细菌外膜的特征分量,是用于检测细菌污染的最佳生物标记,而类脂A是脂多糖的生物活性部分。提取LPS与其类脂A是检测致病菌的基础,对沙门氏菌脂多糖及类脂A的提取与纯化方法进行了研究,结果表明:沙门氏菌LPS产率为1.13%,其中蛋白含量0.85%,核酸含量为1.64%,经SDS-PAGE电泳分析得出提取的为脂多糖,并且杂质较少。将类脂A质谱结果与文献比较,类脂A出峰位置与目前报道的相同,证明提取正确。Salmonella ATCC 13311 as a Gram-negative bacteria,which is the pathogen that causes human and animal intestinal infectious diseases,has serious impact on food safety environment and threaten public health.Lipopolysaccharide(LPS)is best for biomarker detection of bacterial contamination,because it is the characteristic component of the outer membrane of gram-negative bacteria,and lipid A is the bioactive fraction of lipopolysaccharide.LPS extract is the basis of the detection.In this paper,the extraction and purification of Salmonella lipopolysaccharide and lipid A were studied.The results showed that the yield of LPS was 1.13%,the protein content was 0.85%,the nucleic acid content was 1.64%,SDS-PAGE analysis showed that the extract was lipopolysaccharide and had less impurity.Comparing the results of lipid-mass spectrometry with the literature,the position of lipid A was the same as that reported at present,which proved that the extraction was correct.The results show that the purified Salmonella LPS has high purity and little impurity.

关 键 词：沙门氏菌 脂多糖 类脂A 提取纯化 

分 类 号：R318.19[医药卫生—生物医学工程]