便携式MicroRNA快速检测系统  

Portable rapid microRNA detection system

作　　者：王燕飞[1,2,3] 余东升[1,3] 陈海燕 张战盈[1,2,3] 方蔚恺 杨喆[1,3] 陆泽橼 李艳蕾[1,3] 纪玉峰 关一夫 徐赤东[1,3] 江海河 WANG Yan-fei;YU Dong-sheng;CHEN Hai-yan;ZHANG Zhan-ying;FANG Wei-kai;YANG Zhe;LU Ze-yuan;LI Yan-lei;JI Yu-feng;GUAN Yi-fu;XU Chi-dong;JIANG Hai-he(Center of Medical Physics and Technology,Hefei Institutes of Physical Science, Chinese Academy of Sciences,Hefei 230031,China;University of Science and Technology China,Hefei 230026,China;Anhui Province Key Laboratory of Medical Physics and Technology,Hefei 230031,China;China Medical University,Shenyang 110001,China)

机构地区：[1]中国科学院合肥物质科学研究院医学物理与技术中心,安徽合肥230031 [2]中国科学技术大学,安徽合肥230026 [3]医学物理与技术安徽省重点实验室,安徽合肥230031 [4]中国医科大学,辽宁沈阳110001

出　　处：《光学精密工程》2018年第3期541-547,共7页Optics and Precision Engineering

基　　金：国家自然科学基金资助项目(No.81371896)

摘　　要：MicroRNA(miRNA)与疾病的发生和发展密切相关,可作为疾病诊断标志物。常规检测miRNA的方法耗时较长且操作复杂,本文设计了一种便携式miRNA快速检测系统。该系统采用光电检测技术检测滚环扩增后的标志物miRNA受激发出的荧光强度,对采集到的荧光数据进行分析,得出miRNA的变化情况。实验得出便携式miRNA快速检测系统在试剂浓度为0.1~1μmol时,荧光强度线性偏倚不超过±5%,系统重复性大于95%。通过对冠心病检测标志物miR-499进行滚环扩增实验,结果表明设计的便携式miRNA快速检测系统能够快速有效地检测miRNA。MicroRNA(miRNA)is closely related to the occurrence and development of certain diseases and can be used as a diagnostic marker.Conventional methods for the detection of miRNA are time-consuming and complex.Herein,a portable rapid miRNA detection system was designed.This system records the fluorescence intensity of the marker that is amplified via rolling circle amplification using a photoelectric detection technique,and analyzes the fluorescence data to obtain the changes in miRNA.Through our experiments,we observed that for reagent concentrations in the range 0.1-1μmol,the linearity of the fluorescence intensity detected by the miRNA fluorescence detection equipment was less than±5%,and the repeatability was≥95%.Our roll amplification experiment involving the coronary heart disease diagnostic marker,miR-499,confirmed that the portable rapid miRNA detection system can detect miRNA quickly and effectively.

关 键 词：光电检测 荧光检测 弱光探测 miRNA 滚环扩增 

分 类 号：X835[环境科学与工程—环境工程]