加拿大一枝黄花提取物的抗氧化活性研究  

Antioxidant activity of extracts from Solidago canadensis

作　　者：沈校 王振兴 陈丽琼[1,2] 关家怡 邹峥嵘 

机构地区：[1]江西师范大学生命科学学院,南昌330022 [2]江西省亚热带植物资源保护与利用重点实验室,南昌330022 [3]西南林业大学轻工与食品工程学院,昆明650224

出　　处：《广西植物》2018年第3期299-305,共7页Guihaia

基　　金：国家自然科学基金(31760099);国家"十二五"科技支撑计划项目(2011BAC13B04);江西省教育厅科技项目(GJJ14249)~~

摘　　要：为进一步开发和利用加拿大一枝黄花(Solidago canadensis),该研究采用氯化铝显色法和福林酚法测定加拿大一枝黄花乙醇提取物及其不同极性萃取物中的总黄酮和总酚的含量;以抗坏血酸(Vitamin C,Vc)和二丁基羟基甲苯(BHT)为阳性对照,应用2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)、1,1-二苯基苦基苯肼(DPPH)自由基清除体系、铁离子还原能力(FRAP)法和抗氧化能力指数(ORAC)法研究其体外抗氧化活性。结果表明:乙酸乙酯萃取物中的总黄酮(202.45 mg·g^(-1))和总酚(485.94 mg·g^(-1))含量最高,且其抗氧化活性最强,并强于阳性对照Vc(P<0.05)。因而,加拿大一枝黄花乙酸乙酯萃取物将有可能成为一种潜在的天然高效抗氧化剂,具有广泛的应用前景。In order to develop and utilize Solidago canadensis(Solidago),the total flavonoids and phenolic contents in its ethanol extract(70%),and different ploarity extracts were investigated by AlCl 3 assay and Folin-Ciocalteu assay,respectively,and the in vitro antioxidant activities of them were also studied by several methods including ABTS,DPPH,FRAP and ORAC assay,with Vc and BHT as positive control.The results showed that the ethyl acetate extract possessed the highest amount of total flavonoids(202.45 mg·g-1)and phenolic(485.94 mg·g-1),and it had the strongest antioxidant activity than other extracts,even higher than Vc(P<0.05).Thus,the ethyl acetate extract of S.canadensis can be regarded as a kind of potential resource of high-efficiency and natural antioxidants.This will provide the references for further exploiting and utilizing the S.canadensis.

关 键 词：加拿大一枝黄花 提取物 抗氧化活性 

分 类 号：Q946[生物学—植物学]