4株番木瓜根际解磷细菌发酵条件优化研究  

Optimization of Fermentation Conditions of 4 Rhizosphere Phosphate-solubilizing bacteria of Carica papaya

作　　者：吴海波[1] 王樱潼 叶维雁 刘惠民[1] Wu Haibo;Wang Yingtong;Ye Weiyan;Liu Huimin(Key Laboratory of Biodiversity Conservation in the Southwest of the State Forestry Administration,Southwest Forestry University, Kunming Yunnan 650224,China;Guangxi Zhuang Autonomous Region Subtropical Crop Research Institute,Namiing Guangxi 530001,China)

机构地区：[1]西南林业大学国家林业局西南地区生物多样性保育重点实验室,云南昆明650224 [2]广西壮族自治区亚热带作物研究所,广西南宁530001

出　　处：《西南林业大学学报（自然科学）》2018年第2期117-125,共9页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省森林灾害预警与控制重点试验室开放基金(ZK14A101)资助;云南省教育厅科学研究基金(51500617)资助;西南林业大学林学一级学科博士研究生创新基金资助

摘　　要：以番木瓜根际分离出的4株具较好解磷效果菌株为试材,采用单因素实验以及正交实验研究温度、pH、摇床转速、接种量、装液量和培养时间对4株菌株解磷能力的影响。结果表明:4株菌株在初始pH 7.0和发酵培养5 d时均具有最高的解磷能力,而其他4个因素对其解磷能力的影响均表现出差异性。2株无机磷菌的最佳培养温度和接种量均相同,分别为33℃和3 mL,但摇床转速和装液量不同,分别为180、200 r/min和60、50 mL;2株有机磷菌最佳培养温度和摇床转速均相同,分别为31℃和160 r/min,但装液量和接种量不同,分别为60、50 mL和1、4 mL。1n this study,a total of phosphate-solubilizing bacterial colonies were isolated from rhizosphere of Carica papaya.The effects of temperature,pH,shaking speed,inoculum size,liquid volume and culture time on the ability of 4 strains to dissolve phosphorus were studied by single factor experiment and orthogonal experiment.Results showed that under the condition of pH 7.0 and cultured for 5 days,4 bacterial colonies had best phosphate-solubilizing ability,while the other 4 factors had significant difference in the phosphate-solubilizing.2 inorganic phosphate-solubilizing bacterium were the same culture temperature and inoculum density,they were 33℃and 3 mL,but the shaker speed and liquid volume were different respectively,they were 180 r/min and 200 r/min,60 mL and 50 mL.The other 2 organic phosphate-solubilizing bacterium optimal fermentation conditions were the same culture temperature and the same shaker speed,they were 31^and 160 r/min,but the liquid volume and inoculum density were different,they were 60,1 mL and 50,4 mL respectively.

关 键 词：番木瓜 细菌 菌株 解磷能力 发酵条件 

分 类 号：S688.2[农业科学—观赏园艺]