siRNA靶向抗酶抑制剂下调前列腺癌细胞PC3中鸟氨酸脱羧酶表达  

siRNA-mediated antizyme inhibitor down-regulates the expression of ornithine decarboxylase of prostate cancer cell PC3

作　　者：金涛[1] 李倩[2] 欧洁 王艳林[2] 吴红艳[2,3] JIN Tao;LI Qian;OU Jie;WANG Yanlin;WU Hongyan(People s Hospital of Three Gorges University,the First People s Hospital of Yichang,Yichang 443002,China;Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy,Medical College of Three Gorges University,Yichang 443002,China;.Dept.of Immunology,Medical College of Three Gorges University,Yichang 443002,China)

机构地区：[1]三峡大学人民医院(宜昌市第一人民医院) [2]三峡大学医学院肿瘤微环境与免疫治疗湖北省重点实验室 [3]三峡大学医学院免疫学系,湖北宜昌443002

出　　处：《基础医学与临床》2018年第4期475-479,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81402557)

摘　　要：目的探讨抗酶抑制剂(AZIN)对前列腺癌细胞PC3鸟氨酸脱羧酶(ODC)表达水平及增殖的影响。方法将siRNA-AZIN转染前列腺癌PC3细胞,分别用RT-PCR及Western blot检测抗酶(AZ)、AZIN及ODC在mRNA和蛋白水平的表达,MTT检测细胞增殖。结果 siRNA-AZIN转染前列腺癌PC3细胞后,AZIN RNA表达水平降低(P<0.01);PC3细胞AZIN蛋白表达水平降低(P<0.05),且使ODC的表达水平降低(P<0.05);PC3细胞增殖能力明显减弱(P<0.05)。结论 siRNA-AZIN可下调前列腺癌细胞PC3中ODC的表达。Objective To evaluate whether down-regulating antizyme inhibitor(AZIN)can regulate the expression of ornithine decarboxylase(ODC)and the proliferation of prostate cancer cell PC3 or not.Methods siRNA-AZIN transfected prostate cancer cell PC3,the level of antizyme(AZ),AZIN and ODC were measured by RT-PCR and westernblot.MTT was used to measure the proliferation of cells.Results The mRNA level of AZIN declined(P<0.01);the protein level of AZIN and ODC declined(P<0.05).Knockdown of AZIN significantly inhibited the proliferation of PC3(P<0.05).Conclusions Transfecting siRNA-AZIN can decrease the level of AZIN,then the decline level of ODC inhibits the proliferation of PC3.

关 键 词：抗酶抑制剂 鸟氨酸脱羧酶 抗酶 SIRNA 

分 类 号：R730.5[医药卫生—肿瘤]