结直肠癌中RIP2的表达与侵袭性相关性研究  

Analysis of relationships between expression of RIP2 and invasion in colorectal cancer

作　　者：敬国敏 吴立然 樊坤 裴志刚[2] JING Guomin;WU Liran;FAN Kun;PEI Zhigang(Department of Gastrointestinal Surgery,the First Affiliated Hospital of Chongqing Medical University,Qijiang Hospital,Chongqing 401420,China;Department of Pathology,the First Affiliated Hospital of Chongqing Medical University,Qijiang Hospital,Chongqing 401420,China)

机构地区：[1]重庆医科大学附属一院綦江医院胃肠外科,重庆401420 [2]重庆医科大学附属一院綦江医院病理科,重庆401420

出　　处：《国际检验医学杂志》2018年第7期834-836,共3页International Journal of Laboratory Medicine

摘　　要：目的通过检测结直肠癌(CRC)组织中受体相互作用蛋白2(RIP2)的表达水平,并观察调高RIP2水平后肿瘤细胞的侵袭迁移能力,以探讨RIP2在肿瘤细胞侵袭与迁移中的作用。方法采用免疫组织化学法检测CRC组织(48份)及癌旁黏膜组织(56份)中RIP2的表达水平,并应用JetPRIME试剂将pEGFP-RIP2质粒转染入SW480结肠癌细胞株,上调其RIP2表达水平后,观察肿瘤细胞侵袭迁移能力的变化。结果 CRC组织中RIP2表达显著低于癌旁黏膜组织,且差异具有统计意义(P<0.05);上调RIP2后,SW480细胞迁移能力减弱,且差异具有统计意义(P<0.05)。结论 RIP2在CRC组织中表达下降,并与肿瘤细胞侵袭转移相关。Objective To explore the expression of RIP2 in colorectal cancer(CRC)tissues,and the invasion and migration was observed after RIP2 was increased in CRC cell line.Methods 48 cases of CRC paraffin-embedded specimens and 56 cases of tumor-adjacent tissues were collected,and expressions of RIP2 was tested by immunohistochemistry(IHC).pEGFP-RIP2 plasmid and JetPRIME were employed to increase the RIP2 in SW480 cells,and variety of invasion and migration was tested in SW480 cells.Results It was showed that expressions of RIP2 was lower in CRC tumors than in tumor-adjacent tissues(P<0.05).After RIP2 increased,migration ability of SW480 cells weakened.Conclusion The expression of RIP2 was decreased in CRC tissues,and it was closely related to tumor cell′s invasion and metastasis.

关 键 词：结直肠癌 受体相互作用蛋白2 基因干扰 侵袭 

分 类 号：R361[医药卫生—病理学] R446.8[医药卫生—基础医学]