蛹虫草摇瓶菌种培养基的优化  

Optimization of Culture Medium for Shaking Flask of Cordyceps militaris

作　　者：龙海涛 刘标 颜小明 郝继伟[1] LONG Hai-tao;LIU Biao;YAN Xiao-ming(College of Life Science,Linyin University,Linyi,Shandong 276000)

机构地区：[1]临沂大学生命科学学院,山东临沂276000

出　　处：《安徽农业科学》2018年第10期89-91,共3页Journal of Anhui Agricultural Sciences

基　　金：国家级大学生创新创业训练计划项目(201610452023)

摘　　要：[目的]优化蛹虫草摇瓶菌种培养基。[方法]以菌丝体干质量为指标,首先采用单因素试验筛选适于菌丝体生长的最佳碳氮源,再以正交试验优化碳源与氮源以及无机盐与VB1的最佳配比。[结果]蛹虫草优化的摇瓶培养基配方为红薯50 g/L、可溶性淀粉10 g/L、牛肉膏10 g/L、酵母膏10 g/L、KH2PO41.5 g/L、Mg SO4·7H2O 1.0 g/L、VB10.10 g/L,p H自然,利用该配方菌丝干质量可达36.33 g/L。[结论]优化的摇瓶培养基可为后续研究和生产提供基础数据。[Objective]To optimize culture medium for shaking flask of Cordyceps militaris.[Method]Using mycelium dry quality as the index.First,the best carbon and nitrogen source suitable for mycelia growth was selected by single factor test.Then,the optimum ratio of carbon source and nitrogen source,inorganic salt and VB 1 was optimized by orthogonal experiment.[Result]The optimum shaking flask medium for Cordyceps militaris was as follow:sweet potato 50 g/L,soluble starch 10 g/L,beef paste 10 g/L,yeast extract 10 g/L,KH 2PO 4 1.5 g/L,MgSO 4·7H 2O 1.0 g/L,VB 1 0.10 g/L,pH natural.The dry weight of mycelia produced by this formula can reach 36.33 g/L.[Conclusion]The optimizing culture medium for shaking flask of Cordyceps militaris can provide basic data for subsequent research and production.

关 键 词：单因素试验 正交设计 摇瓶培养基 蛹虫草 

分 类 号：S567.35[农业科学—中草药栽培]