苏尼替尼对雌二醇诱发的去卵巢大鼠子宫内膜渗出和血管通透性的干预作用研究  

Intervention effect of Sutveratrol on endometrial exudation and capillary permeability induced by estrogen in ovariectomized rats

作　　者：李长兴[1] 莫晨玲 李建华[1] 孔德霞 李娟 代冬芳[1] 永胜[1] Chang-xing Li;Chen-ling Mo;Jian-hua Li;De-xia Kong;Juan Li;Dong-fang Dai;Sheng Yong(College of Medicine,Qinghai University,Xining,Qinghai 810001,China;The Fourth People’s Hospital of Qinghai Province,Xining,Qinghai 810007,China;The Second People’s Hospital of Xining,Xining,Qinghai 810001,China)

机构地区：[1]青海大学医学院,青海西宁810001 [2]青海省第四人民医院,青海西宁810007 [3]青海省西宁市第二人民医院,青海西宁810001

出　　处：《中国现代医学杂志》2018年第10期8-11,共4页China Journal of Modern Medicine

基　　金：青海省科技厅项目(No:2017-ZJ-708);教育部春晖计划项目(No:Z2015068);国家自然科学基金(No:81060249)

摘　　要：目的探讨苏尼替尼对雌二醇诱发的去卵巢大鼠血管通透性和子宫内膜渗出的干预作用。方法健康雌性SD大鼠随机分为假手术组(Sham组)、卵巢切除组(Ovx组)、卵巢切除+17β-雌二醇组(Ovx+Est组)、卵巢切除+17-β雌二醇+苏尼替尼组(Ovx+Est+Sut组)4组,每组6只。给药21 d后取材。镜下观察子宫内膜形态学变化,用ELISA法检测各组血清血管内皮细胞生长因子(VEGF)的浓度。结果雌二醇诱发去卵巢SD大鼠给予苏尼替尼干预后,子宫重量和子宫系数下降,子宫内膜嗜酸性粒细胞渗出减少,血清VEGF浓度降低。结论苏尼替尼能拮抗雌二醇诱发子宫内膜的渗出,使大鼠子宫内膜增生,血管通透性下降。To explore the intervention effect of Sutveratrol on endometrial leakage and capillary permeability induced by estradiol in ovariectomized rats.Methods Twenty-four adult healthy female Sprague-Dawley(SD)rats were randomly divided into four groups:Sham,Ovx,Ovx+Est and Ovx+Est+Sut groups,with 6 rats in each group.Blood and tissue samples were taken 21 d after medication.The serum VEGF concentration was detected by Sandwich ELISA,and the morphological changes of endometrium were observed under microscope.Results The weight and coefficient of the uterus decreased after intervention with Sutveratrol,and the serum concentration of VEGF and the eosinophil infiltration in endometrium also decreased significantly after medication.Conclusions Sutveratrol antagonizes the estrogen-induced exudation in endometrium,contributes to endometrial hyperplasia,and also leads to the reduction of vascular permeability.

关 键 词：去卵巢大鼠 17Β-雌二醇 苏尼替尼 

分 类 号：R-332[医药卫生]