高海拔地区藏族乳腺浸润性导管癌凋亡相关蛋白的表达及凋亡指数的研究  

Expressions of apoptosis-related proteins and apoptosis index of Tibetan breast invasive ductal cancer in high altitude

作　　者：赞梅[1] 刘五甲 韩静绮[3] 杨娟[1] 白丽艳[2] 张文彦 杜尕金措 祁玉娟[1] Mei Zan;Wu-jia Liu;Jing-qi Han;Juan Yang;Li-yan Bai;Wen-yan Zhang;Jin-cuo Duga;Yu-juan Qi(Qinghai Provincial People’s Hospital,Xining,Qinghai 810007,China;Qinghai University,Xining,Qinghai 810016,China;Affiliated Hospital,Qinghai University,Xining,Qinghai 810001,China)

机构地区：[1]青海省人民医院,青海西宁810007 [2]青海大学,青海西宁810016 [3]青海大学附属医院,青海西宁810001

出　　处：《中国现代医学杂志》2018年第10期43-50,共8页China Journal of Modern Medicine

基　　金：青海省自然科学基金青年项目(No:2012-Z-940Q)

摘　　要：目的探讨藏族乳腺浸润性导管癌Bcl-2、Bax及Caspase 3和凋亡指数(AI)的表达及临床意义。方法采用免疫组织化学法研究60例世居藏族乳腺浸润性导管癌与60例藏族乳腺纤维瘤石蜡组织切片中Bcl-2、Bax和Caspase 3蛋白的表达;采用原位DNA缺口末端标记法检测凋亡指数。结果藏族乳腺浸润性导管癌Bcl-2、Bax及Caspase 3的表达率分别为51.7%、76.7%及60.0%,藏族乳腺纤维瘤组织的Bcl-2、Bax及Caspase 3表达率分别为81.7%、85.0%和80.0%,乳腺浸润性导管癌与乳腺纤维瘤Bcl-2和Caspase 3表达率比较,差异有统计学意义(P<0.05),乳腺纤维瘤组织高于乳腺浸润性导管癌。乳腺浸润性导管癌中AI阳性率与乳腺纤维瘤比较,差异有统计学意义(P<0.05),乳腺浸润性导管癌低于乳腺纤维瘤。AI与肿瘤直径、TNM分期、孕激素受体(PR)表达相关(P<0.05),乳腺浸润性导管癌Bcl-2的表达与淋巴结转移、雌激素受体(ER)表达相关(P<0.05),Caspase 3的表达与ER表达相关(P<0.05);乳腺浸润性导管癌中Bcl-2蛋白表达与Bax、Caspase 3蛋白表达相关(P<0.05)。结论藏族乳腺浸润性导管癌中Bcl-2、Caspase 3蛋白低表达;Bcl-2的表达与Bax、Caspase 3的表达相关;Bcl-2的表达与淋巴结转移、ER表达相关;藏族乳腺浸润性导管癌中AI低表达与凋亡蛋白Bcl-2、Bax、Caspase 3的表达无关;AI与肿瘤直径、TNM分期、PR表达相关。Objective To investigate the expressions of Bcl-2,Bax,caspase 3 and apoptotic index(AI)in Tibetan breast invasive ductal cancer,and analyze their correlations with clinicopathological characteristics.Methods The expressions of Bcl-2,Bax and caspase 3 were examined with immunohistochemical stain in histopathologic slides of 60 cases of Tibetan breast invasive ductal cancers and 60 cases of Tibetan breast fibrocellular tumors,and AI was detected by TUNEL method.Results The positive expression rates of Bcl-2,Bax,caspase 3 were 52.2%,76.7%and 60.0%in Tibetan breast invasive ductal cancer and 81.7%,85.0%and 80.0%in Tibetan fibrocellular tumor separately.The positive expression rates of Bcl-2 and caspase 3 in Tibetan breast invasive ductal cancer were lower than those in Tibetan breast fibroma tissues(P<0.05).The AI of Tibetan breast invasive ductal cancer was lower than that of Tibetan breast fibrocellular tumor tissues(P<0.05).The AI was related to the diameter of tumor,TNM stage and PR in Tibetan breast invasive ductal cancer(P<0.05).Bcl-2 expression in Tibetan breast invasive ductal cancer was related to the lymph node involvement and ER(P<0.05),caspase 3 was related to the ER(P<0.05).Bcl-2 was correlated with Bax and caspase 3 in Tibetan breast invasive ductal cancer(P<0.05).Conclusions The expressions of Bcl-2 and caspase 3 in Tibetan breast invasive ductal cancer are lower than those in Tibetan breast fibrocellular tumor.Bcl-2 expression is correlated with Bax and caspase 3 in Tibetan breast invasive ductal cancer.The expression of Bcl-2 is also related with the lymph node involvement and ER.Apoptotic index is lower in Tibetan breast invasive ductal cancer than in breast fibrocellular tumor.AI has correlations with tumor size,TNM stage and PR,but has no correlation with the expression of Bcl-2,Bax or caspase 3 in Tibetan breast invasive ductal cancer.

关 键 词：Bcl-2 BAX CASPASE3 凋亡指数 乳腺浸润性导管癌 藏族 

分 类 号：R737.9[医药卫生—肿瘤]