大鼠脑缺血再灌注后线粒体ATP酶活性、形态学变化及丹参多酚酸的保护作用  

Effects of Salvianolateon on ATPase activity of mitochondria and pathomorphology in rats with cerebral ischemia reperfusion

作　　者：李富强[1] 王伟[2] 尹金鹏[2] 冯涛[1] 尹晓刚[1] 邓倩[1] 刘荣志[2] 白宏英[3] LI Fuqiang;WANG Wei;YIN Jinpeng(Department of Neurology,The First Affiliated Hospital of NanYang Medical college,Nanyang 473000,China)

机构地区：[1]南阳医学高等专科学校第一附属医院神经内科,河南南阳473000 [2]南阳医学高等专科学校人体解剖学教研室,河南南阳473000 [3]郑州大学第二附属医院神经内科,河南郑州450014

出　　处：《中风与神经疾病杂志》2018年第3期238-241,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的观察注射用丹参多酚酸对大鼠脑缺血再灌注损伤后线粒体ATP酶活性、及形态学的影响。方法将54只雄性SD大鼠随机分成3组(n=18):假手术组(Sham组)、缺血再灌注组(IR组)、丹参多酚酸组(Salvianolate组)。HE染色法观察大脑神经元的组织病理学变化;TTC染色检测脑梗死体积;分光光度计发法线粒体丙二醛含量和线粒体Na^+/K^+ATP酶、Ca^(2+)ATP酶、Mg^(2+)ATP酶活性。结果假手术组大鼠脑组织红染,未见梗死灶;丹参多酚酸组神经细胞变性坏死程度、脑梗死面积较缺血再灌注组明显减轻;与缺血再灌注组相比,丹参多酚酸组线粒体丙二醛含量下降(P<0.05)和线粒体Na^+/K^+ATP酶、Ca^(2+)ATP酶、Mg^(2+)ATP酶活性升高(P<0.05)。结论注射用丹参多酚酸对缺血再灌注损伤有保护作用,其机制与提高线粒体ATP酶活性有关。Objective To explore the effect of Salvianolate on ATPase activity induced by focal cerebral ischemia in rats.Methods 54 male Sprague Dawley(SD)rats were randomly divided into 3 groups(n=18):sham group,ischemia-reperfusion group(I/R)and Salvianolate group.Histopathological changes of cortex neurons were observed under HE staining.Infarct volumes were measured by staining with triphenyltetrazolium chloride(TTC),and activities of Na+/K+ATPase,Ca 2+ATPase,Mg 2+ATPase of mitochondria were measured by spectrophotometer.Result The brain’s cortex of sham group showed red,indicating no infarct.Salvianolate group,compared with the ischemia-reperfusion group,significantly reduced Degeneration of necrotic cells,infarct size(P<0.05).The activities of Na+/K+ATPase,Ca 2+ATPase,Mg 2+ATPase of mitochondria in the Salvianolate group,compared with the ischemia-reperfusion group,were noticeably increased(P<0.05),however,the levels of MDA was decreased(P<0.05).Conclusion Salvianolate has a neuroprotective effect in focal cerebral ischemia rats.The mechanism is associated with Increased ATPase activity of mitochondria.

关 键 词：缺血再灌注 丹参多酚酸 线粒体 ATP酶 

分 类 号：R743.3[医药卫生—神经病学与精神病学]