呕吐毒素对IPEC-J2细胞凋亡的影响  

Effects of Deoxynivalenol on Apoptosis of IPEC-J2 Cells

作　　者：廖美芳[1,2] 孟英才 詹济华[1] 印遇龙 廖鹏[2] 李玲 LIAO Meifang;MENG Yinchai;ZHAN Jihua;YIN Yulong;LIAO Peng;LI Ling(School of Pharmacy,Hunan University of Traditional Chinese Medicine,Changsha 410208,China;Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;Key Laboratory for Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Changsha 410125,China)

机构地区：[1]湖南中医药大学药学院,长沙410208 [2]中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,长沙410125

出　　处：《动物营养学报》2018年第3期1027-1034,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金青年基金(31402088)

摘　　要：本研究以IPEC-J2细胞为材料,研究不同浓度和时间处理下呕吐毒素(DON)对猪空肠上皮细胞凋亡的影响。通过CCK-8试剂盒检测细胞增殖毒性,筛选出合适的DON作用时间(6、12、24 h)。通过低浓度(200 ng/mL)和高浓度(2 000 ng/mL)DON分别作用IPEC-J2细胞6、12、24 h,检测DON对细胞有丝分裂周期和细胞早期凋亡的影响。结果表明:1)DON对IPEC-J2细胞生长有明显抑制作用,200和2 000 ng/mL DON浓度下,48和72 h的细胞存活率显著低于24 h(P<0.05);2 000 ng/mL DON浓度下,72 h的细胞存活率显著低于48 h(P<0.05)。2)不同DON浓度对细胞周期影响不同,低浓度DON主要作用于细胞有丝分裂S期,干扰DNA的复制;而高浓度DON主要作用于细胞有丝分裂G2/M期,干扰有关酶与纺锤丝蛋白质的合成。3)DON作用6 h,低浓度DON组和高浓度DON组早期凋亡率和总凋亡率显著高于对照组(P<0.05);DON作用12 h,高浓度DON组早期凋亡率和总凋亡率显著高于对照组和低浓度DON组(P<0.05)。由此可见,DON抑制了IPEC-J2细胞增殖,低浓度DON使细胞有丝分裂停留在S期,高浓度DON使细胞有丝分裂停留在G2/M期。DON促使细胞早期凋亡,使总凋亡率上升,且促凋亡作用随DON浓度升高而增强。This experiment was conducted to determine the effect of deoxynivalenol(DON)on the apoptosis of IPEC-J2 cells after different concentrations and time treatment.An appropriate exposure time(6,12,and 24 hour)used CCK-8 assay to test for cell proliferation.The effects of DON on cell mitosis cycle and early cell apoptosis were examined by treated the IPEC-J2 cells with low concentration(200 ng/mL)and high concentrations(2 000 ng/mL)DON for 6,12,24 hours.The results showed as follows:1)DON significantly inhibited the growth of IPEC-J2 cells.At 200 and 2 000 ng/mL DON concentrations,the cell survival rate of 48 and 72 h was significantly lower than that of 24 h(P<0.05),and the cell survival rate of 72 h was significantly lower than that of 48 at 2 000 ng/mL DON concentration(P<0.05).2)Different DON concentrations had different effects on cell cycle.The low concentration of DON mainly acted on mitosis stage S and interferes with the replication of DNA,but the high concentration of DON mainly acted on the mitotic phase G2/M and interferes with the synthesis of enzymes and spindle filament proteins.3)DON for 6 hours,the early apoptosis rate and total apoptosis rate of low concentration DON group and high concentration DON group were significantly higher than those of control group(P<0.05);DON for 12 hours,the early apoptosis rate and total apoptosis rate of high concentration DON group were significantly higher than those of control group and low concentration DON group(P<0.05).In conclusion,DON can inhibit IPEC-J2 cells proliferation,low concentration of DON can caused cell mitosis to stay in the S phase,high concentration of DON can caused cell mitosis to stay in the G2/M period.DON also can promote cell apoptosis and increase total apoptosis rate,and this effect is enhanced with DON concentration increasing.

关 键 词：呕吐毒素 IPEC-J2细胞 周期 凋亡 增殖 

分 类 号：S811.2[农业科学—畜牧学]