四株饲用芽孢杆菌的分离鉴定及其16S-rRNA的序列分析  

Isolation and identification of four forage Bacillus and its 16S-rRNA sequence analysis

作　　者：柴俊[1] 朱丽 宋泉芝 刘旭川[2] 张以芳[1] 许琳[1] 普岳红[2] CHAI Jun;ZHU Li;SONG Quanzhi;LIU Xuchuan;ZHANG Yifang;XU Lin;PU Yuehong(Research Center of Animal Products Processing Technology,Yunnan Agricultural University,Kunming,Yunnan Province 650201,China;College of Animal Science and Technology,Yunnan Agricultural University,Kunming,Yunnan Province 650201,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南农业大学省畜产品加工工程技术研究中心,云南昆明650201

出　　处：《中国饲料》2018年第7期20-23,共4页China Feed

基　　金：云南省重点新产品开发计划(2014BB004);云南省院士工作站(2014IC034);福建省家畜传染病防治与生物技术重点室开放基金项目(2016KL05)

摘　　要：芽孢杆菌是一类抗性强耐高温的细菌,部分芽孢杆菌有益生作用,在动物肠道内能有效抑制病原菌,促进肠道有益菌的繁殖,维持动物肠道内的微生态平衡。本试验从引进的饲料样品中分离出4株杆菌,分别命名为YB-1、YB-2、YB-3和YB-4,通过表型鉴定及16S-rRNA测序鉴定,YB-1为苏云金芽孢杆菌、YB-2、YB-4为枯草芽孢杆菌、YB-3为蜡样芽胞杆菌。16S-rRNA序列同源分析结果表明:YB-2、YB-4与枯草芽孢杆菌亚种的同源性分别为99.91%和100%;YB-1与苏云金芽孢杆菌亚种的同源性为99.17%,YB-3与蜡样芽胞杆菌亚种的同源性为100%,从样品中分离出两株枯草芽孢杆菌,一株苏云金芽孢杆菌和一株蜡样芽胞杆菌。Bacillus is a strong resistance to high temperature bacteria,part of them can be used as probiotics,inhibit harmful bacteria in animal intestines effectively,promote the growth of beneficial bacteria in the gut and maintain the ecological balance in the intestines of animals.4 strains of Bacilli separated from the introduction of the feed sample in this experiment,respectively named for YB-1,YB-2,YB-3 and YB-4.By means of 16S-rRNA sequencing identification,the results showed that YB-1 is the B.thuringiensis,YB-2,YB-4 are B.subtilis,YB-3 is B.cereus.16S-rRNA sequence homology analysis showed that the homology of YB-2、YB-4and B.subtilis were 99.91%and 100%,respectively;YB-1and B.thuringiensis was 99.17%,YB-3and B.cereus was 100%.Two isolates of B.subtilis,a B.thuringiensis and a B.cereus isolated from the sample.

关 键 词：芽孢杆菌 分离 鉴定 16S-rRNA 

分 类 号：S816.3[农业科学—饲料科学]