6种胃液游离microRNAs对胃腺癌的诊断价值  

Diagnostic value of six free microRNAs in gastric juice in gastric adenocarcinoma

作　　者：陈妍洁 吴昊[1,2] 董玲 刘韬韬[1,2] 沈锡中 CHEN Yan-jie;WU Hao;DONG Ling;LIU Tao-tao;SHEN Xi-zhong(Department of Gastroenterology,Zhongshan Hospital,Fudan University,Shanghai 20032,China;Shanghai Institute of Liver Diseases,Shanghai 200032,China)

机构地区：[1]复旦大学附属中山医院消化科,上海200032 [2]复旦大学附属中山医院上海肝病研究所,上海200032

出　　处：《中国临床医学》2018年第1期13-17,共5页Chinese Journal of Clinical Medicine

基　　金：上海市青年科技英才扬帆计划(16YF1401500)~~

摘　　要：目的:探讨6种胃液游离microRNAs(以下简称miRNAs)对胃癌的潜在诊断价值。方法:选择2013年于复旦大学附属中山医院进行胃镜检查的53例患者为研究对象。提取胃液并采用实时荧光定量PCR(qRT-PCR)检测其中6种游离miRNAs表达量。按疾病诊断情况将患者分为胃癌组(24例胃腺癌患者)和对照组(16例胃溃疡患者和13例慢性胃炎患者),采用非参数Mann-Whitney检验比较两组患者胃液游离miRNAs的表达差异,采用ROC曲线下面积计算诊断效能。结果:与对照组相比,胃癌组患者胃液中miR-17、miR-18a、miR-20a、miR-21和miR-378的表达差异均有统计学意义(P<0.05)。miR-17、miR-18a、miR-21和miR-378具有中等诊断效能,miR-20a和miR-106b有较低诊断效能。结论:胃液6种游离miRNAs对于胃癌具有潜在诊断价值。Objective:To explore the value of six free microRNAs(miRNAs)in gastric juice in diagnosing gastric adenocarcinoma.Methods:Totally 53 patients who underwent gastroscopy in 2013 were enrolled.The free miRNAs in gastric juice were extracted and detected by quantitative reverse-transcriptase polymerase chain reaction assay(qRT-PCR).The patients were divided into gastric cancer group(24 cases of gastric adenocarcinoma)and control group(16 cases of gastric ulcer and 13 cases of chronic gastritis).The nonparametric Mann-Whitney test was used to compare the miRNAs expression in gastric juice between the two groups and the area under receiver operating characteristic curve was used to evaluate the diagnostic accuracy.Results:All of the six miRNAs had an increased tendency in gastric cancer.Compared with control group,miR-17,-18a,-20a,-21,and-378 were significantly increased in gastric cancer group(all P<0.05),and miR-17,-18a,-21,-378 had a moderately diagnostic value,while miR-20a and-106b had a lowly diagnostic value.Conclusions:Free miRNAs in gastric juice have potential diagnostic value in gastric adenocarcinoma.

关 键 词：MIRNAS 胃腺癌 胃液 诊断 肿瘤标志物 

分 类 号：R735.2[医药卫生—肿瘤]