二烯丙基二硫对人白血病细胞增殖和钙网蛋白表达的影响  

Effect of Diallyl disulfide on cell proliferation and calreticulin expression in human leukemia HL-60 cells

作　　者：陈歆[1] 黄磊[1] 王学锋[2] 尹艳华 熊炜 邓雪冰 阳海华 苏琦[3] 伍世葵[2] CHEN Yin;HUANG Lei;WANG Xuefeng;YIN Yanhua;XIONG Wei;DENG Xuebing;YANG Haihua;SU Qi;WU Shikui(Hunan Traditional Chinese Medical College,Zhuzhou 412012,Hunan China)

机构地区：[1]湖南中医药高等专科学校护理系,湖南株洲412000 [2]湖南中医药高等专科学校附属第一医院 [3]南华大学医学院肿瘤研究所

出　　处：《中南医学科学杂志》2018年第1期23-27,共5页Medical Science Journal of Central South China

基　　金：湖南省卫生与计划委员会课题(C2014-20)

摘　　要：观察二烯丙基二硫(DADS)对人白血病HL-60细胞增殖和钙网蛋白(CRT)表达的影响。DADS(1.25 mg/L)处理HL-60细胞24、48和72 h,siRNA抑制CRT的表达,采用MTT法检测细胞活力,实时定量PCR和Western blot检测CRT的表达。与对照组比较,DADS处理24、48和72 h组HL-6细胞的增殖水平均显著性降低,呈现时间依赖性(均P<0.05)。与对照组比较,siRNA瞬时转染显著降低了CRT mRNA的表达(P<0.05),也显著降低了细胞的增殖水平(P<0.05),与DADS具有协同效应。DADS抑制了HL-60细胞增殖,其机制可能与下调钙网蛋白的表达有关。To observe the effect of Diallyl disulfide(DADS)on the cell proliferation and expression of calreticulin(CRT)in human leukemia HL-60 cells.HL-60 cells were incubated with DADS(1.25mg/L)for 24,48 and 72 h.Expression of CRT was inhibited by siRNA.Cell viability was tested by MTT assay.Expression of CRT was test by real time quantitative PCR and Western bolt.Compared with the control group,the proliferation of HL-60 cells was significantly decreased in time dependent manner after treatment with DADS for 24,48 and 72 h.Compared with the control group,Expression of CRT was significantly decreased by transient transfection of siRNA of CRT.There was a synergistic effect between DADS and siRNA of CRT on expression of CRT.Compared with the control group,the proliferation of HL-60 cells was significantly decreased by siRNA of CRT.There was a synergistic effect between DADS and siRNA of CRT on the proliferation of HL-60 cells.DADS inhibits cell proliferation in human leukemia HL-60 cells,which mechanism may be related with down regulation of CRT expression.

关 键 词：二烯丙基二硫 钙网蛋白 人白血病HL-60细胞 细胞增殖 

分 类 号：R733.4[医药卫生—肿瘤]