丹红注射液对高脂血症大鼠肝脏AMPK/SREBP-1/ACC通路的影响  

Effect of Danhong injection on AMPK/SREBP-1/ACC pathways of liver in hyperlipidemic rats

作　　者：陈娟[1] 汪胡风 邓军[2] 周惠芬 万海同[1] 杨洁红 CHEN Juan;WANG Hu-feng;DENG Jun;ZHOU Hui-fen;WAN Hai-tong;YANG Jie-hong(College of Basic Medical Science,Zhejiang Chinese Medical University,Hangzhou　310053,China;New Drug Safety Evaluation Research Center,Zhejiang Academy of Medical Sciences,Hangzhou　310013,China)

机构地区：[1]浙江中医药大学基础医学院,浙江杭州310053 [2]浙江省医学科学院安全性评价研究中心,浙江杭州310013

出　　处：《中国药理学通报》2018年第4期528-532,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金重点项目(No 81630105);浙江省科技厅公益性技术应用研究计划项目(No 2017C37179);浙江省自然科学基金重点项目(No LZ17H270001);浙江中医药大学基础医学院科技创新团队项目(No JCIT 2016-1);浙江中医药大学科研基金项目(No 2013ZZ02)

摘　　要：目的探讨丹红注射液(Danhong injection,DHI)对大鼠高脂血症的作用及机制。方法采用高脂饲料制备大鼠高脂血症模型,检测各组大鼠肝脏腺苷酸激活蛋白激酶(AMPK)、p-AMPK、胆固醇调节元件结合蛋白(SREBP-1)、乙酰辅酶A羧化酶(ACC)、p-ACC的蛋白表达水平。结果给予丹红注射液后,肝脏AMPK、SREBP-1和ACC的蛋白表达水平明显降低(P<0.05),p-ACC和p-AMPK的蛋白表达水平明显升高(P<0.05)。结论丹红注射液通过增强AMPK的活化,抑制SREBP-1和ACC的活性,促进脂肪酸氧化,减少脂质沉积,有效降低了高脂血症大鼠的血脂水平。Aim To discuss the effect of Danhong injection(DHI)on hyperlipidemia in rats and its possible mechanism.Methods The hyperlipidemia model of rats were induced by high fat diet.The protein expression of adenosine 5’-monophosphate-activated protein kinase(AMPK),p-AMPK,cholesterol-binding element binding protein(SREBP-1),adenylate-activated protein kinase carboxylasecetyl-CoA(ACC)and p-ACC in liver were detected using Western blot.Results The protein expression levels of AMPK,SREBP-1 and ACC significantly decreased(P<0.05),but the protein expression levels of p-ACC and p-AMPK significantly increased(P<0.05).Conclusions Danhong injection can reduce the activity of SREBP-1 and ACC by enhancing the activation of AMPK,and effectively reduce the blood lipid level of hyperlipidemic rats by promoting fatty acid oxidation and reducing lipid deposition.

关 键 词：丹红注射液 AMPK/SREBP-1/ACC通路 高脂血症 脂肪酸氧化 脂质分解 脂肪合成 

分 类 号：R-332[医药卫生] R282.71