rh-aFGF卡波姆940凝胶对1型糖尿病大鼠皮肤创伤的修复作用  

The therapeutic effect of rh-aFGF carbomer 940 gel on wound healing in type Ⅰ diabetic SD rat models

作　　者：杨选鑫 惠琦 曹高忠[2] 刘建国[3] 杜晓霄 李校堃 王晓杰[1] YANG Xuan-xin;HUI Qi;CAO Gao-zhong;LIU Jian-guo;DU Xiao-xiao;LI Xiao-kun;WANG Xiao-jie(College of Pharmacy,Wenzhou Medical University,Wenzhou Zhejiang　325035,China;the First People’s Hospital of Wenzhou Medical University,Wenzhou Zhejiang　325000,China;School of Pharmacy,Second Military Medical University,Shanghai　200433,China;Institute of Biological Sciences,Wenzhou University,Wenzhou Zhejiang　325035,China)

机构地区：[1]温州医科大学药学院,浙江温州325035 [2]温州医科大学附属第一人民医院药剂科,浙江温州325000 [3]第二军医大学药学院,上海200433 [4]温州大学生命科学研究院,浙江温州325035

出　　处：《中国药理学通报》2018年第4期557-562,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81601695);温州市公益性社会发展(医疗卫生)科技资助项目(No Y20150104)

摘　　要：目的研究重组人酸性成纤维细胞生长因子(recombinant human acidic fibroblast growth factor,rh-aFGF)卡波姆940凝胶对糖尿病大鼠皮肤创伤修复作用。方法使用STZ诱导产生SD大鼠糖尿病模型,建立全层皮肤切除和烫伤两种皮肤创伤模型,设置对照组、赋形剂组、90 AU rh-aFGF凝胶组和270 AU rh-aFGF凝胶组4个实验组。用创伤面积和愈合速率评价各组疗效;HE染色观察创面中成纤维细胞、肌成纤维细胞、胶原纤维和毛细血管生长情况;半定量评分评价rh-aFGF诱导的上述指标生长情况。结果 rh-aFGF卡波姆940凝胶明显减少糖尿病SD大鼠全层皮肤切除和烫伤模型的皮肤损伤面积(P<0.05),促进其创伤愈合速率(P<0.05)。HE染色结果表明,rh-aFGF卡波姆凝胶明显促进成纤维细胞和胶原纤维增殖评分(P<0.05)。结论 rh-aFGF卡波姆凝胶可能在一定程度上保护创面微环境和保护rhaFGF发挥生物学活性,促进成纤维细胞增殖和胶原纤维沉积,从而促进糖尿病SD大鼠皮肤创伤愈合过程,有望成为一种治疗糖尿病溃疡的新制剂。Aim To study the therapeutic effect of recombinant human acidic fibroblast growth factor(rh-aFGF)carbomer 940 gel in the treatment of skin wound healing in type I diabetic rats.Methods Two types of skin trauma models,namely,full-thickness wound and scalded wound,were established in a model of type I diabetes mellitus using STZ-induced SD rats.The rats were divided into control group,vehicle group,90 AU rh-aFGF gel group and 270 AU rh-aFGF gel group in each skin wound models.The wound area and wound healing rate were used to evaluate the therapeutic effect.The growth of fibroblasts,fibrocytes,collagen fibers and vessel capillaries in the wound was observed using HE staining and analysed by semi-quantitative score.Results The rh-aFGF carbomer gel significantly reduced the traumatic area as well as promoted the wound healing rate of the skin trauma model of SD rats of type I diabetes mellitus(P<0.05).HE staining showed that rh-aFGF carbomer gel significantly promoted the pathological score of fibroblasts and collagen fibers(P<0.05).Conclusions rh-aFGF carbomer gel might play a protective role in micro-environment of wound and rh-aFGF,which could benefit for proliferation of fibroblasts and collagen,therefore promoting the healing process of skin wound in SD rats with type I diabetes mellitus,and it might be expected to be a new preparation for the treatment of chronic trauma in diabetes mellitus.

关 键 词：重组人酸性成纤维细胞生长因子 糖尿病溃疡 卡波姆940 凝胶制剂 皮肤创伤 愈合率 

分 类 号：R-332[医药卫生] R322.99