L-型钙通道参与大鼠动脉收缩反应的异质性  

L-type calcium channel involved in heterogeneity of arterial constriction in different organs of rats

作　　者：刘林 邝素娟 杨慧 饶芳 张梦珍 麦丽萍 林秋雄 单志新 杨敏 邓春玉 LIU Lin;KUANG Su-juan;YANG Hui;RAO Fang;ZHANG Meng-zhen;MAI Li-ping;LIN Qiu-xiong;SHAN Zhi-xin;YANG Min;DENG Chun-yu(School of Pharmaceutical Sciences,Southern Medical University,Guangzhou　510515,China;Medical Research Center of Guangdong General Hospital;Guangdong Cardiovascular Institute,Guangdong Academy of Medical Sciences,Guangzhou　510080,China)

机构地区：[1]南方医科大学药学院,广东广州510515 [2]广东省人民医院(广东省医学科学院)医学研究部,广东省心血管病研究所,广东广州510080

出　　处：《中国药理学通报》2018年第4期563-569,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No81470440，81273516)

摘　　要：目的研究不同动脉对同一血管收缩剂的反应性是否存在差异,及其与L-型钙通道的关系。方法采用离体血管张力测定实验方法,测定在累积浓度血管收缩剂处理下的大鼠胸主动脉、肾内动脉或冠状动脉的张力变化以及L-型钙通道阻断剂硝苯地平(nifedipine)的影响。结果苯肾上腺素(phenylephrine,Phe)对冠状动脉无明显影响,但可诱导胸主动脉和肾内动脉产生明显的浓度依赖性收缩,胸主动脉的pEC_(50)明显大于肾内动脉(P<0.05);给予硝苯地平孵育后,再给予累积浓度的Phe,胸主动脉收缩的下降幅度大于肾内动脉(P<0.05)。5-羟色胺(5-hydroxy tryptamine,5-HT)对胸主动脉的收缩作用不明显,但可浓度依赖性诱导肾内动脉和冠状动脉收缩;给予硝苯地平孵育后,再给予累积浓度的5-HT,冠状动脉收缩的下降幅度明显大于肾内动脉(P<0.05)。胸主动脉、肾内动脉和冠状动脉均对血栓素A2类似物(9,11-dideoxy-11α,9α-epoxymethanoprostaglandin,U46619)产生浓度依赖性收缩,肾内动脉收缩量效曲线的E_(max)最小(P<0.05),胸主动脉收缩量效曲线pEC_(50)最大(P<0.05)。给予硝苯地平孵育后,再给予累积浓度的U46619,冠状动脉收缩的下降幅度最大,胸主动脉收缩的下降幅度最小。结论大鼠胸主动脉、肾内动脉及冠状动脉对同一血管收缩剂的反应存在异质性,其中L-型钙通道介导的收缩作用也不同。Aim To study whether there was arterial heterogeneity and association with L-type calcium channel(LCC)in different parts of arteries in response to certain vasoconstrictor.Methods The aorta,renal arteries and coronary arteries were dissected from rats.Arterial ring contractions induced by phenylephrine(Phe),5-hydroxyl tryptamine(5-HT)or U46619 in concentration-dependent manner were measured using the Multi Myograph system and the response to nifedipne was observed.Results(1)Phe had no obvious effect on the tension of coronary artery,but induced concentration-dependent vasoconstriction in aorta and renal artery,and pEC 50 of aorta was significantly higher than that of renal artery(P<0.05).The inhibition rate of nifedipine on the aortic contractile responses was significantly higher than that of renal artery(P<0.05).(2)The contraction induced by 5-HT on aorta was not obvious,but was significant on renal artery and coronary artery.The inhibitory rate of nifedipine on coronary artery vasoconstriction was significantly higher than that of renal artery(P<0.05).(3)U46619 could induce aorta,renal artery and coronary artery concentration-dependent contraction,but the E max of them were both higher than that of renal artery(P<0.05).And the pEC 50 of aorta was the largest(P<0.05).Nifedipine significantly inhibited the contraction of aorta,renal artery and coronary artery induced by U46619 with the greatest inhibitory rate on the coronary artery vasoconstriction and minimal inhibition on aortic vasoconstriction.Conclusions The response to certain vasoconstrictor is different among aorta,renal artery and coronary artery in rats,and the contraction mediated by L-type calcium channel is also different.

关 键 词：动脉异质性 L-型钙通道 血管收缩剂 血管张力 胸主动脉 肾内动脉 冠状动脉 

分 类 号：R-332[医药卫生] R322.12