益气活血方和补肾生髓方对脑缺血再灌注大鼠海马和额顶叶皮质Jagged1、Notch4和Hes1蛋白表达的影响  

Influences of Yiqi Huoxue Decotion and Bushen Shengsui Decotion on Related Proteins in Rats with Focal Cerebral Ischemia/Reperfusion

作　　者：徐伟 王丽娜[2] 王键[2] 胡建鹏[2] 何玲[2] 谭辉[2] 王元茂 XU Wei;WANG Lina;WANG Jian;HU Jianpeng;HE Ling;TAN Hui;WANG Yuanmao(Medical Information Engineering Institute,Anhui University of Chinese Medicine,Hefei 230038,China;Key Laboratory of Xin’an Medicine Jointly Developed by Ministry of Education and Anhui Province,Anhui University of Chinese Medicine,Hefei 230038,China)

机构地区：[1]安徽中医药大学医药信息工程学院,安徽合肥230038 [2]安徽中医药大学省部共建新安医学教育部重点实验室,安徽安徽230038

出　　处：《云南中医学院学报》2017年第6期1-4,13,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家科技支撑计划项目(2012BAI26B01);国家自然科学基金(30973692);国家青年科学基金(81603523);安徽中医药大学自然科学研究项目(2015zr015)

摘　　要：目的观察两种中药复方对脑缺血再灌注大鼠海马和额顶叶皮质Jagged1、Notch4和Hes1蛋白表达的影响,探讨其作用机制。方法 0.26mm尼龙线,造成大鼠右侧大脑中动脉阻塞,采用随机分组法,分入假手术组、模型组、益气活血方组和补肾生髓方组。脑缺血2h后,分别于再灌注7d和14d取缺血侧海马和皮质。采用Western Blot检测海马和额顶叶皮质Jagged1、Notch4和Hes1蛋白表达。结果与假手术组比较,模型组Jagged1、Notch4和Hes1蛋白7d和14d表达均显著上调(P<0.05);与模型组比较,益气活血方组和补肾生髓方组Jagged1、Notch4和Hes1蛋白7d和14d表达表达均显著下调(P<0.05);与补肾生髓方组比较,益气活血方组Jagged1、Notch4蛋白7d和14d表达表达均显著下调(P<0.05)。结论益气活血方和补肾生髓方能通过下调Jagged1、Notch4和Hes1蛋白表达,调节Notch信号通路,影响脑缺血后内源性神经干细胞增殖分化,促进其向神经元分化。Objective To investigate the influences of Yiqi Huoxue Decotion and Bushen Shengsui Decotion on protein expressions of Jagged1、Notch4 and Hes1 in hippocampus and frontal cortex of rats with cerebral ischemia reperfusion injury.Methods The rat model of focal cerebral ischemia/reperfusion was established by using suture method for inducing middle cerebral artery occlusion(MCAO),the rats were randomly divided into sham group,model group,Yiqi Huoxue Decotion group(Y group)and Bushen Shengsui Decotion group(B group).After ischemia for 2h and reperfusion,The hippocampus and frontal cortex of ischemic side were taken tissue samples in 7d and 14d.The protein expressions of Jagged1、Notch4 and Hes1 in hippocampus and frontal cortex were detected by using Western Blot.Results The value of Jagged1、Notch4 and Hes1 protein expressions in model group were higher than that in sham group in 7d and 14d(P<0.05),the value of Jagged1、Notch4 and Hes1 protein expressions in Y group and B group were lower than that in model group in 7d and 14d(P<0.05),the value of Jagged1、Notch4 protein expressions are significant difference between Y group and B group in 7d and 14d(P<0.05).Conclusion Yiqi Huoxue Decotion and Bushen Shengsui Decotion can regulate the Notch signaling pathway,affect the proliferation and differentiation of endogenous neural stem cells after cerebral ischemia,make them differentiated into neurons more through reducing protein expressions of Jagged1、Notch4 and Hes1 in hippocampus and frontal cortex.

关 键 词：益气活血方 补肾生髓方 脑缺血再灌注 JAGGED1 NOTCH4 HES1 

分 类 号：R285.5[医药卫生—中药学]