高血糖对骨髓干细胞衰老的影响  

Effect of hyperglycemia on the senility of bone marrow stem cells

作　　者：刘畇 朱艳[2] 盛霞[2] 何琪[2] 魏宏[2] 简蔚泓[2] 宁红红 秦淑兰[2] LIU Yun;ZHU Yan;SHENG Xia(The Third Affiliated Hospital of Nanchang University,Nanchang,330008;The Department of Anatomy,Bengbu Medical College,Bengbu,233030)

机构地区：[1]蚌埠医学院,蚌埠233030 [2]南昌大学第三附属医院,南昌330008

出　　处：《江西医药》2018年第3期221-224,共4页Jiangxi Medical Journal

基　　金：江西省科技计划项目;编号20142BBG70112

摘　　要：目的探讨高血糖对小鼠骨髓干细胞衰老状况的影响。方法采用链脲佐菌素(STZ)诱导糖尿病小鼠模型(糖尿病组),柠檬酸缓冲液注射的健康小鼠为正常对照组,建模成功8周后,测定两组的体质量、血糖,使用免疫磁珠分离法(MACS)收集CD117+骨髓干细胞,荧光共聚焦观察DNA双链断裂(DSB)损伤标志物γ-H2AX的表达。采用Western blot检测γ-H2AX蛋白表达以及衰老相关蛋白p16表达。结果糖尿病组与正常对照组相比体质量明显下降,血糖明显增高(P<0.01)。与正常对照组相比,糖尿病组γ-H2AX的荧光灰度表达明显提高,差异具有统计学意义。另外,与正常对照组相比,糖尿病组骨髓干细胞γ-H2AX以及p16蛋白的表达明显增强。结论高血糖促进骨髓干细胞衰老的发生。Objective To study the effect of hyperglycemia on the senility of CD117+bone marrow cells(BMCs).Methods Streptozotocin(STZ)induced diabetic mice model was used as the DM group,and the control group mice were injected with the citrate buffer solution.After eight weeks,we measured the weight and blood glucose lever of the two groups,and isolated CD117+BMCs from the two groups by magnetic activated cell seperation(MACS).The expression ofγ-H2AX which was the DNA double-strand break Damage marker was observed by the fluorescence confocal.The expression ofγ-H2AX and P16 protein was measured by western blot.Results Compared with the control group,the weight of DM mice was significantly decreased,and the blood glucose lever of DM mice was significantly increased(P<0.01).The fluorescence gray expression ofγ-H2AX in the DM group was significantly higher than that of control group(P<0.01).The expression ofγ-H2AX and P16 protein in the DM group was higher than that in the control group.Conclusion Hyperglycemia promote the senility of bone marrow stem cells.

关 键 词：高血糖 糖尿病 骨髓干细胞 衰老 

分 类 号：R363[医药卫生—病理学]