新型抗菌物质DAPC对变异链球菌粘附作用的影响  

Effect of DAPC on Adhesion of Streptococcus Mutans

作　　者：蒋娟娜 解保生[1] 吴云鼎 周甜 JIANG Juan-na;XIE Bao-sheng;WU Yun-ding;ZHOU tian(Dept.of Prosthodontics,The Affiliated of Stomatological Hospital of Kunming Medical University,Kunming Yunnan 650100;Stomatological Department of Children’s Hospital of Kunming City Affiliated to Kunming Medical University,Kunming Yunnan 650028;Dept.of Stomatological,Kunminig Calmette Hospital,Kunming Yunnan 650011,China)

机构地区：[1]昆明医科大学附属口腔医院修复科,云南昆明650100 [2]昆明医科大学附属儿童医院口腔科,云南昆明650028 [3]昆明市第一人民医院甘美医院口腔科,云南昆明650031

出　　处：《昆明医科大学学报》2018年第3期55-58,共4页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(U0120140798)

摘　　要：目的研究新型抗菌物质新型氨基酸衍生物抗菌物质-焦谷氨酸双癸基二甲基氨基羧酸盐(N,N-di-n-decyl-N,N-dimethyl-ammonium 5-oxopyrrolidine-2-carboxylate,DAPC)对变异链球菌粘附的影响.方法采用2倍稀释法测定DAPC和葡萄糖酸氯己定对变异链球菌的最低抑菌浓度和最低杀菌浓度.在此基础上,以葡萄糖酸氯己定为阳性对照,PBS为阴性对照,结晶紫法测定比较短时浸泡药物后釉质片表面生物膜的形成量.结果DAPC对变异链球菌的MIC为0.003 125 0%,MBC为0.006 250 0%;葡萄糖酸氯己定对变异链球菌的MIC为0.001 562 5%,MBC为0.003 125 0%.2种药物MIC浸泡后生物膜形成量与PBS浸泡组差异无统计学意义(P>0.05).而随着浓度升高,当葡萄糖酸氯己定和DAPC浓度分别为0.12%和0.24%时,实验组生物膜形成量显著低于PBS组(P<0.01),且在24 h后与葡萄糖酸氯己定组差异无统计学意义(P>0.05).结论 DAPC对变异链球菌有明显的抗菌活性,可持续较长时间抑制细菌粘附作用.Objective To analyze the property of the new antibacterial agent(N,N-di-n-decyl-N,N-dimethyl-ammonium 5-oxopyrrolidine-2-carboxylate,DAPC)to prevent Streptococcus mutans’adhesion on tooth surface.Method determine the minimal inhibitory concentration and minimal bactericidal concentration of DAPC and Chlorhexidine gluconate by liquid dilution method.Set the Chlorhexidine gluconate as the positive control,while PBS as the negative control.Use the crystal violet staining to measure the quantity of biofilm.Result The MIC of DAPC on Streptococcus mutans was 0.0031250%and MBC was 0.0062500%.The MIC of Chlorhexidine gluconate was 0.0015625%,while the MBC was 0.0031250%.When concentration of the two antibacterial agents was MIC,the quantity of biofilm have no significance among three groups.However,when concentration of Chlorhexidine gluconate and DAPC were 0.12%and 0.24%respectively,biofilm of experimental group was lower than PBS,and there is no significance between Chlorhexidine gluconate group and DAPC group after 24 hours incubation.Conclusion New antibacterial agent DAPC have significant effect in inhibiting of Streptococcus mutans.And the residual of DAPC on teeth can maintain a long time of antibacterial effect to inhibit Streptococcus mutans’adhesion.

关 键 词：DAPC 葡萄糖酸氯己定 变异链球菌 粘附 

分 类 号：R780.2[医药卫生—口腔医学]