油菜蜂花粉中抗前列腺增生的活性成分  

Anti-Prostatic Hyperplasia Activity and Ingredient of Rape Bee Pollen

作　　者：达热卓玛 饶剑[1] 徐德平[1] DARE Zhuoma;RAO Jian;XU Deping(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2018年第1期38-43,共6页Journal of Food Science and Biotechnology

基　　金：国家科技支撑计划项目(2007BAD83B02)

摘　　要：以油菜蜂花粉为原料,研究其抗良性前列腺增生的活性成分。采用乙醇提取油菜蜂花粉,经不同溶剂萃取,通过诱导BPH模型小鼠研究不同萃取物抗BPH活性。利用各种色谱柱分离,并利用~1H-NMR、^(13)C-NMR、^(135)DEPT-NMR鉴定化学结构。乙酸乙酯萃取物具有显著抗BPH活性(P<0.05)。分离纯化得到了6个单体化合物鉴定为:山奈酚-3-O-β-D吡喃葡萄糖苷、十八碳-9Z,12Z,15Z-三烯酸甘油酯、3'-甲氧基-1'-十八碳-9 Z,12 Z,15 Z三烯酸甘油酯、十八碳-9 Z,12 Z,15 Z-三烯酰基-N-乙基甘油醚、2'-甲氧基-1',3'-十八碳-9 Z,12 Z,15 Z-三烯酸甘油二酯、α-亚麻酸。To research the anti-material of benign prostatic hyperplasia in rape bee pollen,the study is conducted.Ethanol extracts from rape bee-pollen was extracted by different solvent,BPH-induced mouse model is established to study anti-BPH activity.Using a variety of column separation,and the use of 1H-NMR、13C-NMR、135DEPT-NMR to identify the chemical structure.The ethyl acetate extract has significant anti-BPH activity(P<0.05).Further separation and purification of six compounds were obtained.The use of NMR methods identified as:kaempferol-3-O-β-D-glucopyranoside,octadecatrienoic-9 Z,12 Z,15 Z-trienic acid glycerides,3'-methoxy-1'-ten eight carbon-9 Z,12 Z,15 Z-three acid glyceride,stearidonic-9 Z,12 Z,15 Z-trienoyl-N-ethyl glycidyl ether,2'-methoxy-1',3'-octadecenoic-9 Z,12 Z,15 Z-trienic acid diglyceride,α-linolenic acid.

关 键 词：油菜蜂花粉 良性前列腺增生 提取分离 结构鉴定 

分 类 号：R284.2[医药卫生—中药学]